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Antioxidant Activity and Safety Evaluation of Juice Containing Protaetia brevitarsis
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흰점박이꽃무지(Protaetia brevitarsis) 함유 음료의 in vitro 항산화 관련 생리활성효능 및 안전성 검증

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Type
Academic journal
Author
Jae-Hee Park (경남대학교) So Yun Kim (경남대학교) Mingu Kang (거제시 농업기술센터) Minsoo Yoon (거제시 농업기술센터) Yang il Lee (거제시 농업기술센터) Eunju Park (경남대학교)
Journal
The korean Society of Food Science and Nutrition Journal of the Korean Society of Food Science and Nutrition Vol.41 No.1 KCI Accredited Journals SCOPUS
Published
2012.1
Pages
41 - 48 (8page)

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Antioxidant Activity and Safety Evaluation of Juice Containing Protaetia brevitarsis
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The purpose of this study was to assess the antioxidant activity of vegetable extracts (pumpkin, aloe, and artichoke) containing Protaetia brevitarsis (PB) and the clinical and pathological changes in ICR mice after a single oral administration. The total polyphenol (TP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, total radical trapping antioxidant potential (TRAP), oxygen radical absorbance activity (ORAC), and single cell gel electrophoresis assay were done to measure their antioxidant activities. The effect of vegetable extracts containing PB in TP and the ORAC value was significantly higher than those without PB. In addition, all extracts had effective DPPH· scavenging and ABTS·+ scavenging activities. The protective effect of vegetable extracts with/without PB on H2O2-induced DNA damage was found. In a single-dose toxicity study, mortality, body weight, physiological signs, and biochemical analysis were analyzed. Seventy mice were randomly assigned to 7 experimental groups and were administered three vegetable extracts with and without PB (2 g/kg). A full 14 days after administration, no mice mortality was observed in any group. Body weight, physiological signs, and biochemical analysis were never significantly different from those of the control group. Taken together, these findings indicate that vegetable extracts containing PB with antioxidant activities and safety could be applied as medicinal and edible resources in an industrial area.

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UCI(KEPA) : I410-ECN-0101-2013-511-001228732