The purpose of this study was to investigate trajectories of relational aggression in preschool children. The latent growth curve model was used to examine relational aggression in 3 to 5 year olds. The participants were 3-year-old children recruited from preschools and daycare centers. The children"s verbal ability was assessed by interview and teachers completed measurements of negative emotionality and relational aggression. The findings suggest that relational aggression decreased during the preschool years. Gender, language ability, and negative emotionality showed positive effects on the initial level of relational aggression. Moreover, gender and negative emotionality had negative effects, however, language ability had positive effects on the change rate of relational aggression.