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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
초록· 키워드
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Objective : In this study, the immunomodulatory activity of a mixture of wild Panax ginseng and red-mold rice extracts (MPR) on RAW 264.7 macrophage cells in the presence and absence of methotrexate (MTX), an anti-cancer drug, was investigated.
Methods and Results : In the cell viability, MPR showed a significant cell proliferation and inhibited cell regression by red-mold rice (RMR) alone or MTX alone. MPR induced moderate increase in nitric oxide (NO) production. NO production and inducible nitric oxide synthase (iNOS) mRNA expression by LPS decreased after MPR treatment. In addition, MPR slightly induced COX-2 mRNA expression, but it did not affect the expression of COX-2 mRNA by LPS treatment.
In RT-PCR analyses, MPR induced IL-1α, IL-1β, IL-6, and TNF-α mRNA expression, but had no effect on IL-10 and TGF-β, regardless of MTX treatment. Furthermore, MPR did not interfere with the cytotoxicity of MTX against MCF-7 human breast carcinoma cells.
Conclusions : MPR is efficacious in protecting against MTX-induced cell regression as a result of macrophage activation, resulting in induction of cytokine expression, implying that MPR could be considered an adjuvant in MTX-chemotherapy.
Methods and Results : In the cell viability, MPR showed a significant cell proliferation and inhibited cell regression by red-mold rice (RMR) alone or MTX alone. MPR induced moderate increase in nitric oxide (NO) production. NO production and inducible nitric oxide synthase (iNOS) mRNA expression by LPS decreased after MPR treatment. In addition, MPR slightly induced COX-2 mRNA expression, but it did not affect the expression of COX-2 mRNA by LPS treatment.
In RT-PCR analyses, MPR induced IL-1α, IL-1β, IL-6, and TNF-α mRNA expression, but had no effect on IL-10 and TGF-β, regardless of MTX treatment. Furthermore, MPR did not interfere with the cytotoxicity of MTX against MCF-7 human breast carcinoma cells.
Conclusions : MPR is efficacious in protecting against MTX-induced cell regression as a result of macrophage activation, resulting in induction of cytokine expression, implying that MPR could be considered an adjuvant in MTX-chemotherapy.
목차
Introduction
Materials and Methods
Results
Discussion
Acknowledgments
References
참고문헌 (36)
참고문헌 신청
1. [학술지(정기간행물)] - Gorlick R / 1996 / Intrinsic and acquired resistance to methotrexate in acute leukaemia / N Engl J Med 335 : 1041 ~ 1048
2. [학술지(정기간행물)] - Kremer MJ / 2004 / Toward a better understanding of methotrexate / Arthritis Rheum 50 : 1370 ~ 1382
3. [학술지(정기간행물)] - Mizuno M / 1994 / Differences in immunomodulating effects between wild and cultured Panax ginseng / Biochem Biophys Res Commun 200 : 1672 ~ 1678
4. [학술지(정기간행물)] - Gum SI / 2007 / potent protective effect of wild ginseng (Panax ginseng C.A. Meyer) against benzo[α]pyrene-induced toxicity through metabolic regulation of CYP1A1 and GSTs / J Ethnopharmacol 112 : 568 ~ 576
5. [학술지(정기간행물)] - Tsuji K / 1992a / Extraction of hypotensive substance from wheat beni-koji / Nippon: Shokuhin Kogyo Gakkaish 39 : 913 ~ 918
2. [학술지(정기간행물)] - Kremer MJ / 2004 / Toward a better understanding of methotrexate / Arthritis Rheum 50 : 1370 ~ 1382
3. [학술지(정기간행물)] - Mizuno M / 1994 / Differences in immunomodulating effects between wild and cultured Panax ginseng / Biochem Biophys Res Commun 200 : 1672 ~ 1678
4. [학술지(정기간행물)] - Gum SI / 2007 / potent protective effect of wild ginseng (Panax ginseng C.A. Meyer) against benzo[α]pyrene-induced toxicity through metabolic regulation of CYP1A1 and GSTs / J Ethnopharmacol 112 : 568 ~ 576
5. [학술지(정기간행물)] - Tsuji K / 1992a / Extraction of hypotensive substance from wheat beni-koji / Nippon: Shokuhin Kogyo Gakkaish 39 : 913 ~ 918
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UCI(KEPA) : I410-ECN-0101-2014-519-000282196