A Recombinant Plasmodium vivax Apical Membrane Antigen-1 to Detect Human Infection in Iran

Afsaneh Motevalli Haghi; Mohammad Reza Khoramizade; Mehdi Nateghpour; Gholam Hossein Edrissian; Mohammad Reza Eshraghian; Zargham Sepehrizadeh

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자료유형: 학술저널

저자정보: Afsaneh Motevalli Haghi (Tehran University of Medical Sciences) Mohammad Reza Khoramizade (Tehran University of Medical Sciences) Mehdi Nateghpour (Tehran University of Medical Sciences) Gholam Hossein Edrissian (Tehran University of Medical Sciences) Mohammad Reza Eshraghian (Tehran University of Medical Sciences) Zargham Sepehrizadeh (Tehran University of Medical Sciences)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.50 No.1

발행연도: 2012.2

수록면: 15 - 21 (7page)

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In Iran, Plasmodium vivax is responsible for more than 80% of the infected cases of malaria per year. Control interventions for vivax malaria in humans rely mainly on developed diagnostic methods. Recombinant P. vivax apical membrane antigen-1 (rPvAMA-1) has been reported to achieve designing rapid, sensitive, and specific molecular diagnosis. This study aimed to perform isolation and expression of a rPvAMA-1, derived from Iranian patients residing in an endemic area. Then, the diagnostic efficiency of the characterized Iranian PvAMA-1 was assessed using an indirect ELISA method. For this purpose, a partial region of AMA-1 gene was amplified, cloned, and expressed in pET32a plasmid. The recombinant His-tagged protein was purified and used to coat the ELISA plate. Antibody detection was assessed by indirect ELISA using rPvAMA-1. The validity of the ELISA method for detection of anti-P. vivax antibodies in the field was compared to light microscopy on 84 confirmed P. vivax patients and compared to 84 non-P. vivax infected individuals. The ELISA cut-off value was calculated as the mean+2SD of OD values of the people living in malaria endemic areas from a south part of Iran. We found a cut-off point of OD=0.311 that showed the best correlation between the sera confirmed with P. vivax infection and healthy control sera. A sensitivity of 81.0% and specificity of 84.5% were found at this cut off titer. A good degree of statistical agreement was found between ELISA using rPvAMA-1 and light microscopy (0.827) by Kappa analysis.

#Plasmodium vivax #AMA-1 #recombinant antigen #ELISA #microscopy #Iran

참고문헌 (29)

참고문헌 신청

1. [학술지(정기간행물)] - Snow RW / 2005 / The global distribution of clinical episodes of Plasmodium falciparum malaria / Nature 434 : 214 ~ 217

2. [학술지(정기간행물)] - Greenwood B / 2002 / Malaria in 2002 / Nature 415 : 670 ~ 672

3. [학술지(정기간행물)] - Hay SI / 2004 / The global distribution and population at risk of malaria: Past, present, and future / Lancet Infect Dis 4 : 327 ~ 336

4. [학술지(정기간행물)] - Mendis K / 2001 / The neglected burden of Plasmodium vivax malaria / Am J Trop Med Hyg 64 (1-2 suppl) : 97 ~ 106

5. [학술지(정기간행물)] - Edrissian GH / 2006 / Malaria in Iran: past and present situation / Iranian J Parasitol 1 : 1 ~ 14