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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2013.9
- 수록면
- 266 - 269 (4page)
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Hox genes encode transcription factors important for anterior?posterior body patterning at early stages of embryonic development. However, the precise mechanisms by which signal pathways are stimulated to regulate Hox gene expression are not clear. In the previous study, protein kinase B alpha (Akt1) has been identified as a putative upstream regulator of Hox genes, and Akt1 has shown to regulate Gcn5, a prototypical histone acetyltransferase (HAT), in a negative way in mouse embryonic fibroblast (MEF) cells. Since the activity of HAT such as the CBP/p300, and PCAF (a Gcn5 homolog), was down?regulated by Akt through a phosphorylation at the Akt consensus substrate motif (RXRXXS/T), the amino acid sequence of Gcn5 protein was analyzed. Mouse Gcn5 contains an Akt consensus substrate motif as RQRSQS sequence while human Gcn5 does not have it. In order to see whether Akt1 directly binds to Gcn5, immunoprecipitation with anti?Akt1 antibody was carried out in wild?type (WT) mouse embryonic fibroblast (MEF) cells, and then western blot analysis was performed with anti?Akt1 and anti?Gcn5 antibodies. Gcn5 protein was detected in the Akt1 immunoprecipitated samples of MEFs. This result demonstrates that Akt1 directly binds to Gcn5, which might have contributed the down regulation of the 5’ Hoxc gene expressions in wild type MEF cells.
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REFERENCES
참고문헌 (12)
참고문헌 신청
Barber BA / 2010 / Epigenetic control of Hox genes during neurogenesis, development, and disease / Ann Anat 192:261~274
Deschamps J / 2005 / Developmental regulation of the Hox genes during axial morphogenesis in the mouse / Development 132:2931~2942
Drendall CI / 2010 / Purification and characterization of recombinant CH3 domain fragment of the CREBbinding protein / Protein Expr Purif 70:196~205
Favier B / 1997 / Developmental functions of mammalian Hox genes / Mol Hum Reprod 3:115~131
Jin Q / 2011 / Distinct roles of GCN5/PCAF.mediated H3K9ac and CBP/p300.mediated H3K18/27ac in nuclear receptor transactivation / EMBO J 30:249~262
Deschamps J / 2005 / Developmental regulation of the Hox genes during axial morphogenesis in the mouse / Development 132:2931~2942
Drendall CI / 2010 / Purification and characterization of recombinant CH3 domain fragment of the CREBbinding protein / Protein Expr Purif 70:196~205
Favier B / 1997 / Developmental functions of mammalian Hox genes / Mol Hum Reprod 3:115~131
Jin Q / 2011 / Distinct roles of GCN5/PCAF.mediated H3K9ac and CBP/p300.mediated H3K18/27ac in nuclear receptor transactivation / EMBO J 30:249~262
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