지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.12
- 수록면
- 127 - 132 (6page)
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초록· 키워드
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Purpose: Many substances in saliva or oral health care products interact with each other. The aim of this study was to investigate interactions between hyaluronic acid (HA), lysozyme, peroxidase, and glucose oxidase (GO) in enzymatic activities at low pH levels.
Methods: HA (0.5 mg/mL), hen egg-white lysozyme (HEWL, 30 μg/mL), bovine lactoperoxidase(bLPO, 25 μg/mL), and GO (50 μg/mL) were used. The influences of HA, bLPO, and GO on HEWL activity were determined by measuring the turbidity of a Micrococcus lysodeikticus suspension. The influences of HA and HEWL on bLPO activity were determined by the NbsSCN assay, measuring the rate of oxidation of 5-thio-2-nitrobenzoic acid (Nbs) to 5,5´-dithiobis(2-nitrobenzoic acid) (Nbs)2. The influences of HA and HEWL on GO activity were determined by measuring oxidized o-dianisidine production. All experiments were performed at pH 4, 5, and 6.
Results: HA and GO did not affect the enzymatic activity of HEWL at pH 4, 5, and 6. bLPO enhanced the enzymatic activity of HEWL at pH 5 (p<0.05) and pH 6 (p<0.05) significantly. The enzymatic activity of bLPO was not affected by HA and HEWL at pH 4, 5, and 6. HA and HEWL did not affect the enzymatic activity of the GO at pH 4, 5, and 6.
Conclusions: Peroxidase enhances lysozyme activity at low pH, otherwise there were no significant interactions in enzymatic activities between HA, lysozyme, peroxidase, and GO at low pH levels.
Methods: HA (0.5 mg/mL), hen egg-white lysozyme (HEWL, 30 μg/mL), bovine lactoperoxidase(bLPO, 25 μg/mL), and GO (50 μg/mL) were used. The influences of HA, bLPO, and GO on HEWL activity were determined by measuring the turbidity of a Micrococcus lysodeikticus suspension. The influences of HA and HEWL on bLPO activity were determined by the NbsSCN assay, measuring the rate of oxidation of 5-thio-2-nitrobenzoic acid (Nbs) to 5,5´-dithiobis(2-nitrobenzoic acid) (Nbs)2. The influences of HA and HEWL on GO activity were determined by measuring oxidized o-dianisidine production. All experiments were performed at pH 4, 5, and 6.
Results: HA and GO did not affect the enzymatic activity of HEWL at pH 4, 5, and 6. bLPO enhanced the enzymatic activity of HEWL at pH 5 (p<0.05) and pH 6 (p<0.05) significantly. The enzymatic activity of bLPO was not affected by HA and HEWL at pH 4, 5, and 6. HA and HEWL did not affect the enzymatic activity of the GO at pH 4, 5, and 6.
Conclusions: Peroxidase enhances lysozyme activity at low pH, otherwise there were no significant interactions in enzymatic activities between HA, lysozyme, peroxidase, and GO at low pH levels.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
CONFLICT OF INTEREST
REFERENCES
참고문헌 (30)
참고문헌 신청
1. [학술지(정기간행물)] - Almond A / 2007 / Hyaluronan / Cell Mol Life Sci 64 : 1591 ~ 1596
2. [학술지(정기간행물)] - Park MS / 2010 / Rheological properties of hyaluronic acid and its effects on salivary enzymes and candida / Oral Dis 16 : 382 ~ 387
3. [학술지(정기간행물)] - Sakai A / 2007 / Potential role of high molecular weight hyaluronan in the anti-Candida activity of human oral epithelial cells / Med Mycol 45 : 73 ~ 79
4. [학술지(정기간행물)] - Chen WY / 1999 / Functions of hyaluronan in wound repair / Wound Repair Regen 7 : 79 ~ 89
5. [학술지(정기간행물)] - Kang JH / 2011 / Influences of hyaluronic acid on the anticandidal activities of lysozyme and the peroxidase system / Oral Dis 17 : 577 ~ 583
2. [학술지(정기간행물)] - Park MS / 2010 / Rheological properties of hyaluronic acid and its effects on salivary enzymes and candida / Oral Dis 16 : 382 ~ 387
3. [학술지(정기간행물)] - Sakai A / 2007 / Potential role of high molecular weight hyaluronan in the anti-Candida activity of human oral epithelial cells / Med Mycol 45 : 73 ~ 79
4. [학술지(정기간행물)] - Chen WY / 1999 / Functions of hyaluronan in wound repair / Wound Repair Regen 7 : 79 ~ 89
5. [학술지(정기간행물)] - Kang JH / 2011 / Influences of hyaluronic acid on the anticandidal activities of lysozyme and the peroxidase system / Oral Dis 17 : 577 ~ 583
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UCI(KEPA) : I410-ECN-0101-2016-515-001113392