지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.3
- 수록면
- 54 - 61 (8page)
이용수
이 논문의 연구 히스토리 (4)
2015
Development and Verification of Nested PCR Assay for Detection of Tobacco rattle virus in Plant Quarantine
Siwon Lee
,
Jin-Young Lee
,
Su-Heon Lee
외 1명
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2015.03
학술저널
Development and Verification of Nested PCR Assay for Detection of Tobacco rattle virus in Plant Quarantine
Siwon Lee
,
Jin-Young Lee
,
Su-Heon Lee
외 1명
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2015.03
학술저널
2014
Development and Practical Use of RT - PCR for Seed - transmitted Prune dwarf virus in Quarantine
Siwon Lee
,
Yong-Gil Shin
The Plant Pathology Journal
2014.05
학술저널
Development of RT-PCR and Nested PCR Assay for the Detection of Non-reported Five Seed-transmitted Viruses in Quarantine
Siwon Lee
,
Su Jeong Pa
,
k
외 6명
한국미생물학회 학술대회논문집
2014.04
학술대회자료
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초록· 키워드
오류제보하기
Tobacco rattle virus (TRV) is a plant pathogen belonging to the Group IV positive-sense single-stranded RNA viruses. TRV causes disease in various plants (e.g., potato, tomato and tobacco), for which it was classified as a controlled quarantine virus in Korea. This study aimed to develop specific primer sets for the rapid detection of TRV. Two RT-PCR primer sets were developed for specific detection of TRV. Furthermore, nested primer sets were also developed, which is required for high sensitivity detection in plant quarantine. The RT-PCR and nested PCR products had the following sizes: set 5 (1,096→540 bp), and set 7 (878→756 bp), respectively. In addition, a modified positive-control plasmid was also developed for use as a positive control in TRV quarantine. The diagnostic system for TRV detection was verified using samples from Korean quarantine sites for the last five years (2009-2014). A total of 83 cases were detected among various import crops. This system for detection of TRV will continuously contribute to plant quarantine in the future.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS AND DISCUSSION
REFERENCES
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UCI(KEPA) : I410-ECN-0101-2016-475-001317440