Thomas Sternhold and John Hopkins`s The Whole Booke of Psalms has been an important source for the study of English literature, liturgy and psalmody. The 1609 edition of The Whole Booke of Psalms consists of thirty-three Psalm melodies. However, there was no study investigated the use of mode and characteristics of each Psalm melody. This paper traced a short history of English Psalter publication, especially The Whole Booke of Psalms, and presents transcriptions of thirty-three Psalm melodies in modern notation. This study confirms that six Psalm melodies of the 1609 edition were originated from the earlier Genevan Psalter. The Psalm melodies in The Whole Booke of Psalms were in Ionian and Aeolian modes instead of Dorian and Phrygian as found in the Genevan one. While the melodies in the Genevan Psalter were in conjunct motion in most cases, several melodies in The Whole Booke of Psalms were in disjunct motion. Psalms melodies in The Whole Booke of Psalms were referred by the Psalm number in the early English protestant hymnals. For that reason, transcribed Psalm melodies in modern notation will be a valuable resource for further research in early English congregational hymnody as well as English, Scottish and American Psalmody.