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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 1988.6
- 수록면
- 111 - 116 (6page)
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Monoclonal antibodies to Hepatites B surface antigen (HBs Ag) were manufactured for its use in detailed analysis of HBV antigens and epidemiological survey. Balb/c mice showed average 1: 6,400 ELISA titers when they were immunized 2 times with purified HBs Ag and tested at the time of cell fusion. The level of immune response by mice was one of important factors in the determination of cell fusion frequencies and secretions of monoclonal antibodies by fused hybridoma cell lines. Other factors were the properties of myeloma cell lines used molecular weight of polyethylene glycol (PEG) and its reaction time. The potencies of monoclonal antibodies in culture supernatants were variable from 1: 8 depending on hybridoma lines and days after cell fusion. The monoclonsl antibodies in ascitic fluid showed hundred times higher potencies than those in culture fluids when ascitic fluids were formed by the inoculation of Balb/c mice with established hybridoma lines.
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