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논문 기본 정보

자료유형
학술저널
저자정보
Kim, Gye-Won (Biogenetics Lab, Research Laboratories, Dong-A Phamaceutical Co.Ltd) Hong, Sung-Youl (Biogenetics Lab, Research Laboratories, Dong-A Phamaceutical Co.Ltd) Shin, Soon-Cheon (Biogenetics Lab, Research Laboratories, Dong-A Phamaceutical Co.Ltd) Lee, Sung-Hee (Biogenetics Lab, Research Laboratories, Dong-A Phamaceutical Co.Ltd) Kim, Won-Bae (Biogenetics Lab, Research Laboratories, Dong-A Phamaceutical Co.Ltd)
저널정보
대한약학회 Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea 제10권 제1호
발행연도
1987.1
수록면
18 - 24 (7page)

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Mouse monocolonal antibodies to Hepatitis B surface antien (HBsAg) were prepared and their functional capabilities tested by the method of solid phase enzyme linked immuno sorbent assay (ELISA). HBsAg binding studies inicated that one monoclonal antibody 6E-1-1 bound more HBsAg at a faster rate than the other monoclonal antibodies. Also, for the binding inhibition studies with the selected monoclonal antibody 6E-1-1, one monoclonal antibody 8D-3-6 didn't exhibit binding inhibition for HBsAg. Then, a simultaneous ELISA method was developed for the immunodiagnosis of HBsAg. Different combinations of two monoclonal antibodies as solid phase and horseradish peroxidase (HRPO) labeled phase were studied. The combination of monoclonal antibody of higher affinity constant (6E-1-1) immobilized in a solid phase and monoclonal antibody of lower affinity constant (8D-3-6) as a HRPO laeled phase was more sensitive when two monoclonal antibodies of different affinity constants for HBsAg were prepared.

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