지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 1995.12
- 수록면
- 119 - 127 (9page)
이용수
초록· 키워드
오류제보하기
Core, NS3 and NS4 regions of Korean type hepatitis C virus(HCV) genome were expressed and purified as the form of fusion proteins by using a prokaryotic expression-purification system. Core protein, which was expressed up to 20% of total proteins as a B-galactosidase fusion protein in E. coli, was purified by cleavage with cyanogen bromide(CNBr) followed by cation exchange chromatography. Nonstructural proteins, which were up to 25% of total proteins as a soluble maltose binding protein(MBP) fusion protein in E. coli, were purified by affinity chromatography, factor Xa cleavage and gel filtration. The purified Core(CP14), NS3(P27) and NS4(P14) proteins, which were confirmed as a single band of approximate 14Kda, 27Kda and 14Kda respectively by SDS-polyacrylamide gel electrophoresis, showed the strong immunoreactivity with antibodies in sera of Korean chronic hepatitis C patients on immunoblotting analysis.
목차
등록된 정보가 없습니다.
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Protein
대한외과학회 학술대회 초록집
2016 .11
-
소 설사병 바이러스 구조단백질 E2의 대장균 발현
JOURNAL OF BACTERIOLOGY AND VIROLOGY
1994 .06
-
맥아당결합 단백질에 융합된 면역결핍 바이러스 인테그라제의 생산 및 분석
약학회지
1998 .01
-
Hepatitis C Virus E2 외피항원에 대한 단일클론항체의 특성 연구
JOURNAL OF BACTERIOLOGY AND VIROLOGY
1997 .06
-
대장균에서 발현된 한타바이러스 N Protein의 응용
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2004 .06
-
Intracellular Antibody Fragment Against Hepatitis B Virus X Protein Does Not Inhibit Viral Replication
Yonsei Medical Journal
2006 .01
-
IKKγ Facilitates the Activation of NF-κB by Hepatitis C Virus Core Protein
JOURNAL OF BACTERIOLOGY AND VIROLOGY
2018 .09
-
31. 새로운 90 kDa stress protein의 종양에서의 발현에 관한 연구
대한외과학회 학술대회 초록집
1998 .05
-
Expression of Polyhistidine-Containing Fusion Human HepG2 Type Glucose Transport Protein in Spodoptera Cells and Its Purification Using a Metal Affinity Chromatography
대한의생명과학회지
2010 .09
-
Uncovering the Protein Function from Protein Interaction Study
한국실험동물학회 학술발표대회 논문집
2004 .06
-
De novo hepatitis B virus infection after liver transplantation in hepatitis B core-positive recipients using hepatitis B core-negative grafts
한국간담췌외과학회 학술대회지
2015 .09
-
Protein Expression Profile using Two-Dimensional Gel Analysis in Squamous Cervical Cancer Patients
Cancer Research and Treatment
2006 .01
-
Identification of Hepatitis C Virus Core Domain Inducing Suppression of Allostimulatory Capacity of Dendritic Cells
Archives of pharmacal research : a publication of the Pharmaceutical Society of Korea
2002 .01
-
B형 간염 바이러스의 X단백질에 대한 특이항체의 세포 내 발현
Immune Network
2003 .03
-
Differentially Expressed Proteins in Nitric Oxide-Stimulated NIH/3T3 Fibroblasts: Implications for Inhibiting Cancer Development
Yonsei Medical Journal
2015 .01
-
Finding and Characterization of Viral Nonstructural Small Protein in Prospect Hill Virus Infected Cell
JOURNAL OF BACTERIOLOGY AND VIROLOGY
1999 .12
-
Mining Proteins Associated with Oral Squamous Cell Carcinoma in Complex Networks
Asian Pacific journal of cancer prevention : APJCP
2013 .01
-
Expression of Hepatitis C Virus Core Protein in Hepatocytes Does Not Modulate Proliferation or Apoptosis of CD8+ T Cells
Yonsei Medical Journal
2005 .01
-
활막 세포에서 HCV Core 단백에 의한 Interleukin-8 발현 유도
Immune Network
2006 .03
-
사람 혈청내 B형과 C형 간염바이러스에 대한 항체분포
JOURNAL OF BACTERIOLOGY AND VIROLOGY
1997 .12
이 논문의 저자 정보
이 논문과 함께 이용한 논문
최근 본 자료
댓글(0)
0