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자료유형
학술저널
저자정보
Young-Woo Gong Bo-Young Oh (인천보건환경연구원) Hye-young Kim (인천보건환경연구원) Mi-yeon Lee (인천보건환경연구원) Yong-Hee Kim (인천보건환경연구원) Jong-Myoung Go (인천보건환경연구원) Jea-Mann Lee (인천보건환경연구원) Hye-sook Jeong (국립보건연구원) Doo-Sung Cheon (국립보건연구원)
저널정보
대한바이러스학회 JOURNAL OF BACTERIOLOGY AND VIROLOGY Journal of Bacteriology and Virology 제38권 제4호
발행연도
2008.12
수록면
249 - 257 (9page)

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Noroviruses (NoVs) cause major epidemic gastroenteritis in humans. To obtain the molecular epidemiological information on gastroenteritis sporadic cases in Incheon city, Korea, we analyzed the nucleotide sequences of NoV strains detected during 2005~2007. We performed one step RT-PCR amplifying the open reading frame (ORF) 2 (capsid region) followed by semi-nested PCR from the stool samples from acute gastroenteritis patients from 2005 to 2007. Amplicons of the capsid region of norovirus strains were sequenced and analyzed using MegAlign in DNAstar software. Faecal samples were collected from 6,618 acute gastroenteritis patients during the study period. The incidence of NoV infection was about 10.7% (n=708) among patients with acute gastroenteritis and genotypes of the 320 positive samples were determined by sequence analysis. Sequence comparison of NoV strains revealed that 16 genotypes of GII NoV strains were circulated in Incheon city, from 2005 to 2007. Among norovirus strains, the most prevalent genotype GII/4 was most common 69.7% (223 strains), followed by GII/3 17.2% (55 strains), GII/12 4.4% (14 strains), GII/1 2.2% (7 strains), GII/5 1.6% (5 strains), GII/15 1.3% (4 strains) and 0.6% (2 strains) each of GII/9 and GII/16. The GII-3 strains were most frequently detected in Incheon, 2005. From the phylogenetic analysis of NoV strains, we detected 16 genotypes of GII NoV strains during 2005~2007 in Incheon. Our results suggest that various genotypes of human NoV strains in sporadic case of AGE were circulated in Incheon, Korea.

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