Sequence Variation in Superoxide Dismutase Gene of Toxoplasma gondii among Various Isolates from Different Hosts and Geographical Regions

Shuai Wang; Aiping Cao; Xun Li; Qunli Zhao; Yuan Liu; Hua Cong; Shenyi He; Huaiyu Zhou

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자료유형: 학술저널

저자정보: Shuai Wang (Shandong University) Aiping Cao (Shandong University) Xun Li (Shandong University) Qunli Zhao (Shandong University) Yuan Liu (Shandong University) Hua Cong (Shandong University) Shenyi He (Shandong University) Huaiyu Zhou (Shandong University)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.53 No.3

발행연도: 2015.6

수록면: 253 - 258 (6page)

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Toxoplasma gondii, an obligate intracellular protozoan parasite of the phylum Apicomplexa, can infect all warm-blooded vertebrates, including humans, livestock, and marine mammals. The aim of this study was to investigate whether superoxide dismutase (SOD) of T. gondii can be used as a new marker for genetic study or a potential vaccine candidate. The partial genome region of the SOD gene was amplified and sequenced from 10 different T. gondii isolates from different parts of the world, and all the sequences were examined by PCR-RFLP, sequence analysis, and phylogenetic reconstruction. The results showed that partial SOD gene sequences ranged from 1,702 bp to 1,712 bp and A + T contents varied from 50.1% to 51.1% among all examined isolates. Sequence alignment analysis identified total 43 variable nucleotide positions, and these results showed that 97.5% sequence similarity of SOD gene among all examined isolates. Phylogenetic analysis revealed that these SOD sequences were not an effective molecular marker for differential identification of T. gondii strains. The research demonstrated existence of low sequence variation in the SOD gene among T. gondii strains of different genotypes from different hosts and geographical regions.

#Toxoplasma gondii #PCR-RFLP #superoxide dismutase #sequence variation #phylogenetic analysis

참고문헌 (26)

참고문헌 신청

[학술지(정기간행물)] - Montoya JG / 2004 / Toxoplasmosis / Lancet 363 : 1965 ~ 1976

[학술지(정기간행물)] - Weiss LM / 2009 / Toxoplasmosis: a history of clinical observations / Int J Parasitol 39 : 895 ~ 901

[학술지(정기간행물)] - Cenci-Goga BT / 2011 / Toxoplasma in animals, food, and humans: an old parasite of new concern / Foodborne Pathog 8 : 751 ~ 762

[학술지(정기간행물)] - Sibley LD / 2008 / Population structure of Toxoplasma gondii: clonal expansion driven by infrequent recombination and selective sweeps / Annu Rev Microbiol 62 : 329 ~ 351

[학술지(정기간행물)] - Sibley LD / 1992 / Virulent strains of Toxoplasma gondii comprise a single clonal lineage / Nature 359 : 82 ~ 85

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