지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2015.10
- 수록면
- 1,275 - 1,278 (4page)
이용수
초록· 키워드
오류제보하기
A self-contained cartridge and an integrated instrument for the automated molecular testing are described. The proposed cylindrical cartridge consists of multiple chambers containing all necessary reagents such as specimens, lysis, wash, and elution buffers. The system provides a chain of automatic processes for the molecular test of sample preparation, amplification, and detection. Sample preparation can be fully conducted inside the cartridge, which includes reagents mixing, nucleic acids capturing, wastes-out, and eluates collection. The system also implements the amplifying and detecting processes of the extracted DNA using the peltier heating and photometry scanner, respectively. All flow movements in the instrument are driven by pneumatic actuations of syringe/piston sets and valve controls based on the motor driving. The molecular tests in the system were evaluated clinically using Salmonella bacteria. The performance of the system was verified successfully by comparing with the results from commercial equipment. The system including the disposable cartridge has the potentials to be applicable for point-of-care testing of molecular diagnostics such as Polymerase Chain Reaction (PCR) or other amplification methods such as isothermal amplification, resulting in an early diagnosis of disease.
목차
Abstract
1. INTRODUCTION
2. DESCRIPTION OF THE POC INSTRUMENT
3. RESULTS
4. CONCLUSIONS
REFERENCES
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UCI(KEPA) : I410-ECN-0101-2016-569-001911547