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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 288 - 293 (6page)
이용수
초록· 키워드
오류제보하기
Fully automated system for sample preparation in molecular diagnostics was developed. The small cartridge with multiple chambers and a rotating valve was designed for automated processes such as extraction, separation and purifications during the DNA sample preparation. The automated processes such as injection, mixing, capturing and valve on/off motions were controlled by an affordable, motorized control system. We demonstrated the automated DNA extraction of pathogenic bacteria using this system. The deviations in the purity and real-time polymerase chain reaction (PCR) detection of the automated DNA extraction samples were clearly decreased. The device provided rapid, reliable, and reproducible sample preparation in comparison with manual DNA extraction. This automated platform can be utilized in emergent point-of-care testing of infectious disease and foodborne bacteria.
목차
등록된 정보가 없습니다.
참고문헌 (16)
참고문헌 신청
O’Connor, L. / 2010 / Recent advances in the development of nucleic acid diagnostics / Expert Rev. Med. Devices 7:529~539
Gubala, V. / 2012 / Point-of-care diagnostics: status and future / Anal. Chem 84:487~515
Chin, C. D. / 2012 / Commercialization of microfluidic point-of-care diagnostic devices / Lab Chip 12:2118~2134
Berry, S. M. / 2011 / One-step purification of nucleic acid for gene expression analysis via Immiscible Filtration Assisted by Surface Tension (IFAST) / Lab Chip 11:1747~1753
Byrnes, S. / 2013 / A portable, pressure driven, room temperature nucleic acid extraction and storage system for point-of-care molecular diagnostics / Anal. Methods 5:3177~3184
Gubala, V. / 2012 / Point-of-care diagnostics: status and future / Anal. Chem 84:487~515
Chin, C. D. / 2012 / Commercialization of microfluidic point-of-care diagnostic devices / Lab Chip 12:2118~2134
Berry, S. M. / 2011 / One-step purification of nucleic acid for gene expression analysis via Immiscible Filtration Assisted by Surface Tension (IFAST) / Lab Chip 11:1747~1753
Byrnes, S. / 2013 / A portable, pressure driven, room temperature nucleic acid extraction and storage system for point-of-care molecular diagnostics / Anal. Methods 5:3177~3184
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