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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.3
- 수록면
- 66 - 72 (7page)
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초록· 키워드
오류제보하기
Chinese hamster ovary (CHO) cells have been widely used for production of various recombinant proteins such as cytokines and monoclonal antibodies. The cell aggregation and cell death in CHO cell culture directly affect cell viability, and productivity and quality of products. In this study, we investigated preventing effects of storage-protein 2 (SP2) derived from silkworm hemolymph on cell aggregation and cell death in CHO cell culture producing albumin-erythropoietin (Alb-EPO). The viable cell density in the culture supplemented with 2 mg/mL SP2 was 1.71-fold higher than that in control culture. Increased titer of Alb-EPO was also found in the culture with SP2. Morphology of CHO cells in SP2 supplemented cultures did not differ from that of control. In addition, the cell aggregation rate of the SP2 cultures was reduced 20% compared to the control. Finally, we confirmed that the apoptosis was strongly suppressed by addition of SP2 in the cultures. These results clearly demonstrate that SP2 can be served as an effective supplement for enhancing titer of Alb-EPO via reducing cell aggregation and cell death.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHOD
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (29)
참고문헌 신청
Butler, M. (2005) Animal cell cultures: Recent achievements and perspectives in the production of biopharmaceuticals. Appl. Microbiol. Biotechnol. 68: 283-291.
Li, F., N. Vijayasankaran, A. Shen, R. Kiss, and A. Amanullah (2010) Cell culture processes for monoclonal antibody production. mAbs. 2: 466-479.
Li, J., C. L. Wong, N. Vijayasankaran, T. Hudson, and A. Amanul- lah (2012) Feeding lactate for CHO cell culture processes: Impact on culture metabolism and performance. Biotechnol. Bioeng. 109: 1173-1186.
Chen, P. and S. W. Harcum (2005) Effects of amino acid additions on ammonium stressed CHO cells. J. Biotechnol. 117: 277-286.
Chuan, K. H., S. F. Lim, L. Martin, C. Y. Yun, S. O. H. Loh, F. Lasne, and Z. Song (2006) Caspase activation, sialidase release and changes in sialylation pattern of recombinant human erythropoietin produced by CHO cells in batch and fed-batch cultures. Cytotechnology. 51: 67-79.
Li, F., N. Vijayasankaran, A. Shen, R. Kiss, and A. Amanullah (2010) Cell culture processes for monoclonal antibody production. mAbs. 2: 466-479.
Li, J., C. L. Wong, N. Vijayasankaran, T. Hudson, and A. Amanul- lah (2012) Feeding lactate for CHO cell culture processes: Impact on culture metabolism and performance. Biotechnol. Bioeng. 109: 1173-1186.
Chen, P. and S. W. Harcum (2005) Effects of amino acid additions on ammonium stressed CHO cells. J. Biotechnol. 117: 277-286.
Chuan, K. H., S. F. Lim, L. Martin, C. Y. Yun, S. O. H. Loh, F. Lasne, and Z. Song (2006) Caspase activation, sialidase release and changes in sialylation pattern of recombinant human erythropoietin produced by CHO cells in batch and fed-batch cultures. Cytotechnology. 51: 67-79.
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