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논문 기본 정보
- 자료유형
- 학술대회자료
- 저자정보
- 발행연도
- 2021.10
- 수록면
- 664 - 664 (1page)
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Mammalian cell culture is most common industrial process for the production of recombinant proteins. However, higher cell density and titer are still limited due to factors such as excessive metabolites production, nutrients limitation, and high osmolality. Lactate is the major product from glucose metabolism, and lactate accumulation has been demonstrated to inhibit growth rate and negatively affect antibody production in mammalian cells. To partly overcome this factor, we investigated a simple method using pH control in Chinese hamster ovary (CHO) cells. We performed CHO-DG44 fed-batch cultures under pH conditions (6.9 and 7.0) using rocking bioreactor. As a result, cells grown under low pH condition produced lactate approximately 70% lower than cells grown under high pH condition. We also found that peak viable cell density (PVCD) and final antibody titer were increased in fed-batch culture under low pH condition. Taken together, our results demonstrate that fed-batch culture under pH of 6.9 improves the PVCD and titer in CHO cell culture. ... 전체 초록 보기
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