Rosuvastatin Induces ROS-mediated Apoptosis in Human Prostate Cancer PC-3 Cells

Hyeun Deok Choi; Jong Jin Baik; Sang Hun Kim; Sun Nyoung Yu; Sung Hak Chun; Young Wook Kim; Hyo Won Nam; Kwang Youn Kim; Soon Cheol Ahn

Rosuvastatin Induces ROS-mediated Apoptosis in Human Prostate Cancer PC-3 Cells

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Rosuvastatin이 유도하는 ROS가 전립선암 PC-3 세포주의 세포사멸 유도에 미치는 영향

Type: Academic journal

Author: Hyeun Deok Choi (부산대학교) Jong Jin Baik (부산대학교) Sang Hun Kim (부산대학교) Sun Nyoung Yu (부산대학교) Sung Hak Chun (부산대학교) Young Wook Kim (부산대학교) Hyo Won Nam (부산대학교) Kwang Youn Kim (대구한의대학교) Soon Cheol Ahn (부산대학교)

Journal: Korean Society Of Life Science Journal of Life Science Vol.26 No.4 KCI Accredited Journals

Published: 2016.4

Pages: 398 - 405 (8page)

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Rosuvastatin Induces ROS-mediated Apoptosis in Human Prostate Cancer PC-3 Cells

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Statins, the inhibitors of 3-hydroxy 3-methylglutaryl coenzyme A (HMG-CoA) reductase, are widely used in treatments of hypercholesterolemia and newly known as anti-cancer effect of various cancer cells. Recently, several studies suggested that reactive oxygen species (ROS) play a critical role on cell death signaling. However, mechanism of ROS by rosuvastatin is currently unclear. This study aimed to explore the molecular mechanism of apoptosis by rosuvastatin in human prostate cancer PC-3 cells. Cell viability and apoptosis-related protein expression were measured by MTT assay and western blotting, respectively. In addition, the levels of apoptosis and ROS were analyzed. The results showed that rosuvastatin dramatically reduced cell viability in a dose- and time-dependent manner. We confirmed that rosuvastatin induced apoptosis through reduction of procaspase-3 and cleavage of poly (ADP-ribose) polymerase (PARP) in PC-3 cells. In addition, rosuvastatin stimulated ROS production in a dose-dependent manner and pre-treatment with N-acetylcysteine (NAC), a ROS scavenger, significantly recovered rosuvastatin-induced ROS and apoptosis. Thus, we concluded that rosuvastain induces apoptosis through generation of ROS in human prostate cancer PC-3 cells and provides a promising approach to improve the efficacy of cancer therapy.

#Apoptosis #human prostate cancer PC-3 cells #N-acetylcysteine #reactive oxygen species #rosuvastatin

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Cafforio, P., Dammacco, F., Gernone, A. and Silvestris, F. 2005. Statins activate the mitochondrial pathway of apoptosis in human lymphoblasts and myeloma cells. Carcinogenesis 26, 883-891.

Cardwell, C. R., Mc Menamin, Ú., Hughes, C. M. and Murray, L. J. 2015. Statin use and survival from lung cancer: A population-based cohort study. Cancer Epidemiol. Biomarkers Prev. 24, 833-841.

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최현덕

Affiliation 부산대학교

Fields Humanities > Philosophy > General Philosophy Natural Science > Other Natural Science

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