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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.4
- 수록면
- 25 - 30 (6page)
- DOI
- 10.17519/apiculture.2016.04.31.1.25
이용수
초록· 키워드
오류제보하기
Stonebrood and Chalkbrood are major fungal diseases in honeybee (Apis mellifera), caused by Aspergillus flavus, or by Ascosphaera apis, respectively. Because of similar shapes and symptoms of infected larvae by them, it could be hard to distinguish between both without gene analysis. In this study, we developed a Loop-mediated isothermal amplification (LAMP) method for the specific detection of A. flavus in honeybee. Optimal conditions for A. flavus-specific LAMP were sequentially established using the specific primer sets, RT-A. flavus-F3/ B3/ FIP1 /BIP2. The existence of A. flavus among genomic DNA from honeybee was detected by A. flavus-specific LAMP within 27 minutes, and the detection limit was estimated 105 DNA molecules of target DNA. Specific amplification of A. flavus-specific LAMP can be recognized with colorimetric dyes by naked eyes. It makes easy screening and time-saving to identify A. flavus-infection in field tests. This assay would be useful not only for A. flavus detection in honeybee in laboratory, but also could be applied in apiary field.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULT
DISCUSSION
LITERATURE CITED
참고문헌 (13)
참고문헌 신청
Hong, S.B., S.J. Go, H.D. Shin, J.C. Frisvad and R.A. Samson. 2005. Polyphasic taxonomy of Aspergillus fumigatus and related species.Mycologia, 97(6): 1316-1329.
Lee, H.M., S.H. Nam, J.S. Ha, Y.H. Jo and B.S. Yoon. 2004. PCR Detection Method of Ascosphera apis, Aspergillus flavus for Rapid Identification of Fungal Disease in Honeybee. Korean Journal of Apiculture. 19: 139- 148.
Lee, J.S., S.J. Yong, H.Y. Lim and B.S. Yoon. 2015. A Simple and Sensitive Gene-Based Diagnosis of Aspergillus flavus by Loop-Mediated Isothermal Amplification in Honeybee. Journal of Apiculture, 30(1): 53-59.
Notomi, T., H. Okayama, H. Masubuchi, T. Yonekawa, K. Watanabe, N. Amino and T. Hase. 2000. Loopmediated isothermal amplification of DNA. Nucleic acids research,28(12): e63-e63.
Samson, R.A., C.M. Visagie, J. Houbraken, S.B. Hong, V. Hubka, C.H. Klaassen, ... & J. Varga. 2014. Phylogeny, identification and nomenclature of the genus Aspergillus. Studies in mycology,78: 141-173.
Lee, H.M., S.H. Nam, J.S. Ha, Y.H. Jo and B.S. Yoon. 2004. PCR Detection Method of Ascosphera apis, Aspergillus flavus for Rapid Identification of Fungal Disease in Honeybee. Korean Journal of Apiculture. 19: 139- 148.
Lee, J.S., S.J. Yong, H.Y. Lim and B.S. Yoon. 2015. A Simple and Sensitive Gene-Based Diagnosis of Aspergillus flavus by Loop-Mediated Isothermal Amplification in Honeybee. Journal of Apiculture, 30(1): 53-59.
Notomi, T., H. Okayama, H. Masubuchi, T. Yonekawa, K. Watanabe, N. Amino and T. Hase. 2000. Loopmediated isothermal amplification of DNA. Nucleic acids research,28(12): e63-e63.
Samson, R.A., C.M. Visagie, J. Houbraken, S.B. Hong, V. Hubka, C.H. Klaassen, ... & J. Varga. 2014. Phylogeny, identification and nomenclature of the genus Aspergillus. Studies in mycology,78: 141-173.
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UCI(KEPA) : I410-ECN-0101-2016-527-002869657