지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.4
- 수록면
- 53 - 59 (7page)
- DOI
- 10.17519/apiculture.2015.04.30.1.53
이용수
초록· 키워드
오류제보하기
Stonebrood is a well-known fungal disease of the honeybee. This disease is caused by several species of the fungus Aspergillus. One of these species is Aspergillus flavus. In Korea 2013, Stonebrood disease showed a 13% infection rate in diseased honeybees, but current hive management lacks a rapid field detection system for this fungus. In this study, for easy detection of A. flavus, an A. flavus-specific loop-mediated isothermal amplification detection method was developed. A. flavus-LAMP employs Bst DNA polymerase and a set of four specially designed primers (A. flavus-LAMP F3, B3, FIP, and RIP) which recognize six distinct genetic sequences in A. flavus 18S rRNA gene. As a result, A. flavus was successfully amplified under isothermal conditions at about 59°C within 1 hour. The amplified A. flavus-specific LAMP products were visualized by adding fluorescent dye, SYBR Green I or GeneFinderTM enabling observation with the naked eye under daylight without electrophoresis. This method is expected to be used for field detection of A. flavus.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
LITERATURE CITED
참고문헌 (17)
참고문헌 신청
1. [학술지(정기간행물)] - Alexandra. K / 2007 / Importance of pollinators in changing landscapes for world crops / Proc Biol Sci 274 (1608) : 303 ~ 313
2. [학술지(정기간행물)] - Allen, M.F. / 1996 / The incidence and world distribution of honey bee viruses / Bee World 77 : 141 ~ 162
3. [학술지(정기간행물)] - 조용호 / 2007 / Loop-mediated Isothermal Amplification (LAMP)을 이용한 꿀벌의 Black Queen Cell Virus (BQCV) 신속 검출법의 개발 / Journal of Apiculture 22 (2) : 139 ~ 4
4. [학술지(정기간행물)] - Fujiyuki. T / 2006 / Prevalence and Phylogeny of Kakugo Virus, a Novel Insect Picorna-Like Virus That Infects the Honeybee (Apis mellifera L.), under Various Colony Conditions / Journal of Virology : 11528 ~ 11538
5. [학술지(정기간행물)] - Jie Luo / 2014 / Application of loop-mediated isothermal amplification assays for direct identification of pure cultures of Aspergillus flavus, A. nomius, and A. caelatus and for their rapid detection in shelled Brazil nuts / International Journal of Food Microbiology 172 : 5 ~ 12
2. [학술지(정기간행물)] - Allen, M.F. / 1996 / The incidence and world distribution of honey bee viruses / Bee World 77 : 141 ~ 162
3. [학술지(정기간행물)] - 조용호 / 2007 / Loop-mediated Isothermal Amplification (LAMP)을 이용한 꿀벌의 Black Queen Cell Virus (BQCV) 신속 검출법의 개발 / Journal of Apiculture 22 (2) : 139 ~ 4
4. [학술지(정기간행물)] - Fujiyuki. T / 2006 / Prevalence and Phylogeny of Kakugo Virus, a Novel Insect Picorna-Like Virus That Infects the Honeybee (Apis mellifera L.), under Various Colony Conditions / Journal of Virology : 11528 ~ 11538
5. [학술지(정기간행물)] - Jie Luo / 2014 / Application of loop-mediated isothermal amplification assays for direct identification of pure cultures of Aspergillus flavus, A. nomius, and A. caelatus and for their rapid detection in shelled Brazil nuts / International Journal of Food Microbiology 172 : 5 ~ 12
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Development of in-field-diagnosis of Aspergillus flavus by Loop-Mediated Isothermal Amplification in Honeybee
Journal of Apiculture
2016 .04
-
Isolation of Soil Actinobacterial Strains Showing Antifungal Activity against Aspergillus flavus, a Causative Agent of Stonebrood Disease in Honeybee
Journal of Apiculture
2015 .04
-
꿀벌의 석고병, 백묵병 현장 진단을 위한 신속 Ascosphaera apis 및 Aspergillus flavus 검출법 개발
Journal of Apiculture
2016 .04
-
The Development of Rapid Detection Method against Ascosphaera apis and Aspergillus flavus for On-site Diagnosis of Chalkbrood and Stonebrood in Honey bee
한국양봉학회 학술대회 자료집
2016 .03
-
Metconazole susceptibility tests and development of transformation system in Aspergillus flavus field strains
한국농약과학회 학술발표대회 논문집
2021 .11
-
Antifungal and antiaflatoxigenic natural compounds against Aspergillus flavus
한국농약과학회 학술발표대회 논문집
2015 .10
-
Inhibitory effects of three monoterpenes from ginger essential oil on growth and aflatoxin production of Aspergillus flavus and their gene regulation in aflatoxin biosynthesis
Applied Biological Chemistry
2018 .01
-
Isolation of Antifungal Lactic Acid Bacteria (LAB) from “Kunu” against Toxigenic Aspergillus flavus
Preventive Nutrition and Food Science
2017 .06
-
Develpment of control method in American foulbrood disease (AFB), Stonebrood, and Chalkbrood by Using Actinobacterial Culture
한국양봉학회 학술대회 자료집
2015 .03
-
Development of a loop-mediated isothermal amplification assay for living modified canola GT73
Plant Biotechnology Reports
2022 .08
-
Method validation for quantitative analyzing aflatoxin productivity in Aspergillus sp. isolated from soybean paste
Food Science and Preservation
2023 .02
-
다중PCR증폭과 특이 DNA-chip을 활용한 꿀벌 주요 11종 병원체의 검출법 개발
Journal of Apiculture
2016 .06
-
Development of a diagnostic method for human enteric Adenovirus-41 with rapid, specific and high sensitivity using the loop-mediated isothermal amplification assay
Korean Journal of Agricultural Science
2020 .09
-
Development of a Loop-mediated Isothermal Amplification (LAMP) for Easy Detection of Nosema apis in Honeybee : Does Nosema apis exist in Korea?
한국양봉학회 학술대회 자료집
2015 .03
-
Development of loop-mediated isothermal amplification (LAMP) assay for detecting Vibrio anguillarum using groEL gene
한국수산과학회 양식분과 학술대회
2017 .05
-
Development of a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of Plum pox virus isolated from peach in Korea
한국농약과학회 학술발표대회 논문집
2018 .10
-
Application of groEL gene for species-specific detection of Vibrio anguillarum and Vibrio parahaemolyticus by loop-mediated isothermal amplification (LAMP) assay
한국수산과학회 양식분과 학술대회
2017 .05
-
On-site detection of Salmonella enterica subsp. enterica serovar Thompson by real-time fluorescence loop-mediated isothermal amplification (LAMP) and colorimetric LAMP in meat products
한국식품영양과학회 학술대회발표집
2023 .10
-
Development of diagnostic method for human Astrovirus with rapid, specific and high sensitivity using loop-mediated isothermal amplification method
Korean Journal of Agricultural Science
2020 .03
-
꿀벌 6종 주요 병원체에 대한 초고속 다중 PCR 검출법의 개발
Journal of Apiculture
2017 .04
이 논문의 저자 정보
이 논문과 함께 이용한 논문
Development of Specific Detection Method for Fungal Pathogens in Honeybee by Loop-Mediated Isothermal Amplification
Joo-seong Lee
,
Hee-Young Lim
,
Byoung-Su Yoon
Journal of Apiculture
2013
.10
Biological Synthesis of Manganese Dioxide Nanoparticles Using Kalopanax pictus Plant Leaf Extract
Sun A MOON
,
Bipinchand
,
a SALUNKE
외 1명
한국생물공학회 학술대회
2014
.04
Effect of Sulfurization on CIGS Thin Films by RF Magnetron Sputtering Using a Cu(In1-xGax)Se2 Single Target
Sung Hee Jung
,
Chee Won Chung
한국진공학회 학술발표회초록집
2013
.02
GLOBAL EXISTENCE FOR VOLTERRA-FREDHOLM TYPE FUNCTIONAL IMPULSIVE INTEGRODIFFERENTIAL EQUATIONS
V. VIJAYAKUMAR
,
K. ALAGIRI PRAKASH
,
R. MURUGESU
JOURNAL OF THE KOREAN SOCIETY FOR INDUSTRIAL AND APPLIED MATHEMATICS
2013
.03
유한요소해석을 이용한 환봉 단조공정 최적화
최성기
,
천명식
,
문영훈
소성·가공
2004
.02
최근 본 자료
댓글(0)
0