Increased Expression of TGF-β1 in Correlation with Liver Fibrosis during Echinococcus granulosus Infection in Mice

Yumei Liu; Gulizhaer Abudounnasier; Taochun Zhang; Xuelei Liu; Qian Wang; Yi Yan; Jianbing Ding; Hao Wen; Delixiati Yimiti; Xiumin Ma

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저자정보: Yumei Liu (First Affiliated Hospital of Xinjiang Medical University) Gulizhaer Abudounnasier (First Affiliated Hospital of Xinjiang Medical University) Taochun Zhang (First Affiliated Hospital of Xinjiang Medical University) Xuelei Liu (First Affiliated Hospital of Xinjiang Medical University) Qian Wang (First Affiliated Hospital of Xinjiang Medical University) Yi Yan (First Affiliated Hospital of Xinjiang Medical University) Jianbing Ding (First Affiliated Hospital of Xinjiang Medical University) Hao Wen (First Affiliated Hospital of Xinjiang Medical University) Delixiati Yimiti (Xinjiang Medical University) Xiumin Ma (First Affiliated Hospital of Xinjiang Medical University)

저널정보: 대한기생충학열대의학회 Parasites, Hosts and Diseases The Korean Journal of Parasitology Vol.54 No.4

발행연도: 2016.8

수록면: 519 - 525 (7page)

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To investigate the potential role of transforming growth factor (TGF)-β1 in liver fibrosis during Echinococcus granulosus infection, 96 BALB/c mice were randomly divided into 2 groups, experimental group infected by intraperitoneal injection with a metacestode suspension and control group given sterile physiological saline. The liver and blood samples were collected at days 2, 8, 30, 90, 180, and 270 post infection (PI), and the expression of TGF-β1 mRNA and protein was determined by real-time quantitative RT-PCR and ELISA, respectively. We also evaluated the pathological changes in the liver during the infection using hematoxylin and eosin (H-E) and Masson staining of the liver sections. Pathological analysis of H-E stained infected liver sections revealed liver cell edema, bile duct proliferation, and structural damages of the liver as evidenced by not clearly visible lobular architecture of the infected liver, degeneration of liver cell vacuoles, and infiltration of lymphocytes at late stages of infection. The liver tissue sections from control mice remained normal. Masson staining showed worsening of liver fibrosis at the end stages of the infection. The levels of TGF-β1 did not show significant changes at the early stages of infection, but there were significant increases in the levels of TGF-β1 at the middle and late stages of infection (P<0.05). RT-PCR results showed that, when compared with the control group, TGF-β1 mRNA was low and comparable with that in control mice at the early stages of infection, and that it was significantly increased at day 30 PI and remained at high levels until day 270 PI (P<0.05). The results of this study suggested that increased expression of TGF-β1 during E. granulosus infection may play a significant role in liver fibrosis associated with E. granulosus infection.

#Echinococcus granulosus #TGF-β1 #Liver fibrosis

참고문헌 (21)

참고문헌 신청

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