지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.12
- 수록면
- 569 - 574 (6page)
이용수
초록· 키워드
오류제보하기
BACKGROUND/OBJECTIVES: We investigated the anti-osteoarthritic effects of deer bone extract on the gene expressions of matrix metalloproteinases (MMPs) and collagen type II (COL2) in interleukin-1β-induced osteoarthritis (OA) chondrocytes.
MATERIALS/METHODS: Primary rabbit chondrocytes were treated as follows: CON (PBS treatment), NC (IL-1β treatment), PC (IL-1β + 100 μg/mL glucosamine sulphate/chondroitin sulphate mixture), and DB (IL-1β + 100 μg/mL deer bone extract).
RESULTS: The results of the cell viability assay indicated that deer bone extract at doses ranging from 100 to 500 μg/mL inhibits cell death in chondrocytes induced by IL-1β. Deer bone extract was able to significantly recover the mRNA expression of COL2 that was down-regulated by IL-1β (NC: 0.79 vs. DB: 0.87, P < 0.05) and significantly decrease the mRNA expression of MMP-3 (NC: 2.24 vs. DB: 1.75) and -13 (NC: 1.28 vs. DB: 0.89) in OA chondrocytes (P < 0.05).
CONCLUSIONS: We concluded that deer bone extract induces accumulation of COL2 through the down-regulation of MMPs in IL-1β-induced OA chondrocytes. Our results suggest that deer bone extract, which contains various components related to OA, including chondroitin sulphate, may possess anti-osteoarthritic properties and be of value in inhibiting the pathogenesis of OA.
MATERIALS/METHODS: Primary rabbit chondrocytes were treated as follows: CON (PBS treatment), NC (IL-1β treatment), PC (IL-1β + 100 μg/mL glucosamine sulphate/chondroitin sulphate mixture), and DB (IL-1β + 100 μg/mL deer bone extract).
RESULTS: The results of the cell viability assay indicated that deer bone extract at doses ranging from 100 to 500 μg/mL inhibits cell death in chondrocytes induced by IL-1β. Deer bone extract was able to significantly recover the mRNA expression of COL2 that was down-regulated by IL-1β (NC: 0.79 vs. DB: 0.87, P < 0.05) and significantly decrease the mRNA expression of MMP-3 (NC: 2.24 vs. DB: 1.75) and -13 (NC: 1.28 vs. DB: 0.89) in OA chondrocytes (P < 0.05).
CONCLUSIONS: We concluded that deer bone extract induces accumulation of COL2 through the down-regulation of MMPs in IL-1β-induced OA chondrocytes. Our results suggest that deer bone extract, which contains various components related to OA, including chondroitin sulphate, may possess anti-osteoarthritic properties and be of value in inhibiting the pathogenesis of OA.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (0)
참고문헌 신청함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Effects of gangliosides from deer bone extract on the gene expressions of matrix metalloproteinases and collagen type II in interleukin-1β-induced osteoarthritic chondrocytes
Nutrition Research and Practice
2016 .12
-
Adipose-Derived Stem Cells Suppress Inflammation Induced by IL-1beta through Down-Regulation of P2X7R Mediated by miR-373 in Chondrocytes of Osteoarthritis
Molecules and Cells
2017 .03
-
MiR-29a and MiR-140 Protect Chondrocytes against the Anti-Proliferation and Cell Matrix Signaling Changes by IL-1beta
Molecules and Cells
2016 .02
-
In vitro 및 in vivo 퇴행성관절염 모델에서 오미자 에탄올 추출물에 의한 matrix metalloproteinases의 생성 억제
생명과학회지
2017 .10
-
Carpomitra costata Extract Suppresses Interleukin-1β-Induced Inflammatory Response in SW1353 Human Chondrocytes through Suppressing NF-κB Signaling Pathway
한국해양바이오학회지
2020 .12
-
Low-Molecular-Weight Collagen Peptide Ameliorates Osteoarthritis Progression through Promoting Extracellular Matrix Synthesis by Chondrocytes in a Rabbit Anterior Cruciate Ligament Transection Model
Journal of Microbiology and Biotechnology
2021 .10
-
NFI-C Is Required for Epiphyseal Chondrocyte Proliferation during Postnatal Cartilage Development
Molecules and Cells
2020 .01
-
참당귀 추출분말이 in vitro and in vivo model에서 MMPs 조절 기전
분석과학
2015 .12
-
Effect of Hijikia fusiforme extracts on degenerative osteoarthritis in vitro and in vivo models
Nutrition Research and Practice
2016 .06
-
Effect of Hijikia fusiforme extracts on degenerative osteoarthritis in vitro and in vivo models
Nutrition Research and Practice
2016 .06
-
Role of Long Intergenic Nonprotein-Coding RNA 00511 in Nod-Like Receptor Protein Pyrin Domain 3-Induced Chondrocyte Pyroptosis via the MicroRNA-9-5p/FUT1 Axis
Journal of Microbiology and Biotechnology
2024 .07
-
Cardamonin Inhibited IL-1β Induced Injury by Inhibition of NLRP3 Inflammasome via Activating Nrf2/NQO-1 Signaling Pathway in Chondrocyte
Journal of Microbiology and Biotechnology
2021 .01
-
7α,25-Dihydroxycholesterol-Induced Oxiapoptophagic Chondrocyte Death via the Modulation of p53-Akt-mTOR Axis in Osteoarthritis Pathogenesis
Molecules and Cells
2023 .04
-
Oleanolic Acid Promotes Neuronal Differentiation and Histone Deacetylase 5 Phosphorylation in Rat Hippocampal Neurons
Molecules and Cells
2017 .07
-
흑생강 추출물의 CHON-001 세포에서의 IL-1β로 유도된 염증과 MMPs 발현 억제 효과
생명과학회지
2024 .08
-
Cigarette Smoke Extract Enhances IL-17A-Induced IL-8 Production via Up-Regulation of IL-17R in Human Bronchial Epithelial Cells
Molecules and Cells
2018 .03
-
서식지 환경에 따른 고라니의 행동생태 연구
국립공원연구지
2021 .11
-
Survey of Oxolinic Acid-Resistant Erwinia amylovora in Korean Apple and Pear Orchards, and the Fitness Impact of Constructed Mutants
The Plant Pathology Journal
2022 .10
-
Generalized net model of Osteoarthritis diagnosing
Advanced Studies in Contemporary Mathematics
2017 .01
-
Study of the density of horns from cervids
Journal of species research
2015 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2017-594-001876313