지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.12
- 수록면
- 221 - 230 (10page)
이용수
초록· 키워드
오류제보하기
The rotavirus nonstructural glycoprotein, NSP4, has been identified as the first viral enterotoxin capable of inducing diarrhea. To investigate the biological function of NSP4 in the inflammatory process, a cDNA from human rotavirus (Wa strain) RNA segment 10 was amplified by RT-PCR, cloned into TA vector, and subsequently subcloned into pET23b expression plasmid. The expression of NSP4 protein was determined by SDS-PAGE and Western blotting, then, the protein was purified by affinity chromatography on Ni-NTA-agarose column. The inflammatory effects of NSP4, namely, production of nitric oxide (NO), pro-inflammatory cytokines (IL-1β, IL-6, IL-10, and TNF-α), and prostaglandin E2 (PGE<SUB>2</SUB>), was evaluated using NSP4-stimulated RAW 264.7 murine macrophages and compared with those observed after stimulation with lipopolysaccharide (LPS). The levels of IL-1β, IL-6, and TNF-α were significantly increased, and those of NO and PGE<SUB>2</SUB> also increased in NSP4-stimulated RAW 264.7 cells. These findings indicate that NSP4 plays an important role in the inflammatory response observed during rotavirus infection.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (36)
참고문헌 신청
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[학술지(정기간행물)] - Parashar UD / 2004 / Global mortality associated with rotavirus disease among children in 2004 / J Infect Dis 200 (Suppl 1) : S9 ~ S15
[학술지(정기간행물)] - Hertel PM / 2012 / Rotavirus and biliary atresia: can causation be proven? / Curr Opin Gastroenterol 28 : 10 ~ 17
[학술지(정기간행물)] - Ball JM / 1996 / Agedependent diarrhea induced by a rotaviral nonstructural glycoprotein / Science 272 : 101 ~ 104
[학술지(정기간행물)] - Dong Y / 1997 / The rotavirus enterotoxin NSP4 mobilizes intracellular calcium in human intestinal cells by stimulating phospholipase C-mediated inositol 1,4,5-trisphosphate production / Proc Natl Acad Sci U S A 94 : 3960 ~ 3965
[학술지(정기간행물)] - Parashar UD / 2004 / Global mortality associated with rotavirus disease among children in 2004 / J Infect Dis 200 (Suppl 1) : S9 ~ S15
[학술지(정기간행물)] - Hertel PM / 2012 / Rotavirus and biliary atresia: can causation be proven? / Curr Opin Gastroenterol 28 : 10 ~ 17
[학술지(정기간행물)] - Ball JM / 1996 / Agedependent diarrhea induced by a rotaviral nonstructural glycoprotein / Science 272 : 101 ~ 104
[학술지(정기간행물)] - Dong Y / 1997 / The rotavirus enterotoxin NSP4 mobilizes intracellular calcium in human intestinal cells by stimulating phospholipase C-mediated inositol 1,4,5-trisphosphate production / Proc Natl Acad Sci U S A 94 : 3960 ~ 3965
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UCI(KEPA) : I410-ECN-0101-2017-475-001976841