지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.4
- 수록면
- 90 - 96 (7page)
이용수
초록· 키워드
오류제보하기
BACKGROUND/OBJECTIVES: Although the antioxidative effects of lycopene are generally known, the molecular mechanisms underlying the anti-inflammatory properties of lycopene are not fully elucidated. This study aimed to examine the role and mechanism of lycopene as an inhibitor of inflammation.
METHODS/MATERIALS: Lipopolysaccharide (LPS)-stimulated SW 480 human colorectal cancer cells were treated with 0, 10, 20, and 30 μM lycopene. The MTT assay was performed to determine the effects of lycopene on cell proliferation. Western blotting was performed to observe the expression of inflammation-related proteins, including nuclear factor-kappa B (NF-κB), inhibitor kappa B (IκB), mitogen-activated protein kinase (MAPK), extracellular signal-related kinase (ERK), c-jun NH2-terminal kinase (JNK), and p38 (p38 MAP kinase). Real-time polymerase chain reaction was performed to investigate the mRNA expression of tumor necrosis factor α (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). Concentrations of nitric oxide (NO) and prostaglandin E₂ (PGE₂) were determined via enzyme-linked immunosorbent assays.
RESULTS: In cells treated with lycopene and LPS, the mRNA expression of TNF-α, IL-1β, IL-6, iNOS, and COX-2 were decreased significantly in a dose-dependent manner (P < 0.05). The concentrations of PGE₂ and NO decreased according to the lycopene concentration (P < 0.05). The protein expressions of NF-κB and JNK were decreased significantly according to lycopene concertation (P < 0.05).
CONCLUSIONS: Lycopene restrains NF-κB and JNK activation, which causes inflammation, and suppresses the expression of TNF-α, IL-1β, IL-6, COX-2, and iNOS in SW480 human colorectal cancer cells.
METHODS/MATERIALS: Lipopolysaccharide (LPS)-stimulated SW 480 human colorectal cancer cells were treated with 0, 10, 20, and 30 μM lycopene. The MTT assay was performed to determine the effects of lycopene on cell proliferation. Western blotting was performed to observe the expression of inflammation-related proteins, including nuclear factor-kappa B (NF-κB), inhibitor kappa B (IκB), mitogen-activated protein kinase (MAPK), extracellular signal-related kinase (ERK), c-jun NH2-terminal kinase (JNK), and p38 (p38 MAP kinase). Real-time polymerase chain reaction was performed to investigate the mRNA expression of tumor necrosis factor α (TNF-α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2). Concentrations of nitric oxide (NO) and prostaglandin E₂ (PGE₂) were determined via enzyme-linked immunosorbent assays.
RESULTS: In cells treated with lycopene and LPS, the mRNA expression of TNF-α, IL-1β, IL-6, iNOS, and COX-2 were decreased significantly in a dose-dependent manner (P < 0.05). The concentrations of PGE₂ and NO decreased according to the lycopene concentration (P < 0.05). The protein expressions of NF-κB and JNK were decreased significantly according to lycopene concertation (P < 0.05).
CONCLUSIONS: Lycopene restrains NF-κB and JNK activation, which causes inflammation, and suppresses the expression of TNF-α, IL-1β, IL-6, COX-2, and iNOS in SW480 human colorectal cancer cells.
목차
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (38)
참고문헌 신청
Korean Statistical Information Service. Cause of death [Internet]. Daejeon: Statistics Korea; c2014 [cited 2016 August 29]. Available from: http://kosis.kr/statHtml/statHtml.do?orgId=101&tblId=DT_1B3 4E01&conn_path=I2.
Korean Statistical Information Service. Cancer registration statistics [Internet]. Daejeon: Statistics Korea; c2013 [cited 2016 August 29]. Available from: http://kosis.kr/statHtml/statHtml.do?orgId=117&tblId =DT_117N_A00022&conn_path=I2.
Kim DH. Risk factors of colorectal cancer. J Korean Soc Coloproctol 2009;25:356-62.
Coussens LM, Werb Z. Inflammation and cancer. Nature 2002; 420:860-7.
Tuan J, Chen YX. Dietary and lifestyle factors associated with colorectal cancer risk and interactions with microbiota: fiber, red or processed meat and alcoholic drinks. Gastrointest Tumors 2016;3:17-24.
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Anti-inflammatory effect of lycopene in SW480 human colorectal cancer cells
Nutrition Research and Practice
2017 .04
-
RAW 264.7 대식세포주에서 나노입자화 리코펜의 항염증 증진 효과
Journal of Nutrition and Health
2015 .12
-
Lycopene 유도체를 이용한 Lycopene 색소의 안정성 연구
한국분석과학회 학술대회
2017 .05
-
Effect of saccharin on inflammation in 3T3-L1 adipocytes and the related mechanism
Nutrition Research and Practice
2020 .04
-
Effect of saccharin on inflammation in 3T3-L1 adipocytes and the related mechanism
Nutrition Research and Practice
2020 .04
-
Aster tataricus 물 추출물의 mitogen-activated protein kinase 신호 전달 경로를 통한 면역 조절 효과
Journal of Nutrition and Health
2020 .10
-
Anti-Inflammatory Activity of Oligomeric Proanthocyanidins Via Inhibition of NF-ΚB and MAPK in LPS-Stimulated MAC-T Cells
Journal of Microbiology and Biotechnology
2020 .01
-
A simple method to determine lycopene in solid supplementary food preparations using saponification and liquid chromatography
한국분석과학회 학술대회
2022 .11
-
A simple method to determine lycopene in solid supplementary food preparations using saponification and liquid chromatography
한국분석과학회 학술대회
2022 .11
-
Anti-Inflammatory Effects of Hydroethanolic Extract from Ehretia asperula on Lipopolysaccharide-Stimulated RAW264.7 Macrophages
Journal of Microbiology and Biotechnology
2024 .06
-
LPS로 자극된 RAW 264.7 세포에서 찹쌀떡버섯 균사체로 생물전환된 루모라고사리 추출물의 항염증 효과
생명과학회지
2023 .06
-
RAW 264.7 세포에서 조팝나무 에탄올 분획물의 항염 활성
Journal of Nutrition and Health
2022 .02
-
Dispersion and characterization of environmentally friendly water soluble lycopene pigment particles
한국분석과학회 학술대회
2017 .11
-
Anti-inflammatory effects of proanthocyanidin-rich red rice extract via suppression of MAPK, AP-1 and NF-κB pathways in Raw 264.7 macrophages
Nutrition Research and Practice
2016 .06
-
Anti-inflammatory effects of proanthocyanidin-rich red rice extract via suppression of MAPK, AP-1 and NF-κB pathways in Raw 264.7 macrophages
Nutrition Research and Practice
2016 .06
-
Reconsideration of Classical Antibiotic Lincomycin: Anti-inflammatory Effect in LPS-stimulated RAW 264.7 Cells
한국미생물·생명공학회지
2020 .01
-
RAW 264.7 세포에서의 단풍잎돼지풀 추출물의 항염증 활성 검증
한국미생물·생명공학회지
2020 .01
-
LPS 유도 RAW 264.7 세포와 마우스 모델에서 참치(Katsuwonus pelamis) 유의 항염증 효과
한국미생물·생명공학회지
2015 .01
-
Anti-inflammatory activity of a sulfated polysaccharide isolated from an enzymatic digest of brown seaweed Sargassum horneri in RAW 264.7 cells
Nutrition Research and Practice
2017 .02
-
Anti-inflammatory activity of a sulfated polysaccharide isolated from an enzymatic digest of brown seaweed Sargassum horneri in RAW 264.7 cells
Nutrition Research and Practice
2017 .02
이 논문의 저자 정보
최근 본 자료
댓글(0)
0
UCI(KEPA) : I410-ECN-0101-2017-594-002341064