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논문 기본 정보

자료유형
학술저널
저자정보
박성민 (조선대학교) Lavanya Madhavaraj (조선대학교) 김시욱 (조선대학교)
저널정보
한국생물공학회 KSBB Journal KSBB Journal Vol.32 No.2 (Wn.161)
발행연도
2017.6
수록면
124 - 132 (9page)
DOI
10.7841/ksbbj.2017.32.2.124

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초록· 키워드

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A methane-oxidizing bacterium was isolated from rice paddy field soil around Jeollanam-do province, Korea, and characterized. The isolate was gram-negative, orange pigmented and short rod (1.1-1.2 × 1.6-1.9 μm). It was catalase and urease-negative but oxidase-positive. The strain utilized methane and methanol as sole carbon and energy sources. It had an ability to grow with an optimum pH 7.0 and an optimum growth temperature 30℃. The strain was resistant to antibiotic polymyxin B but sensitive to streptomycin, kanamycin, ampicillin, chloramphenicol and rifampicin. The isolate required copper for their growth with concentration range of 2-25 μM, with an optimum of 10 μM. Under optimal culture condition, specific cell growth rate and generation time were found to be 0.046 hr<SUP>-1</SUP> and 15.13 hr, respectively. Phylogenetic analysis based on 16S rDNA sequences indicated that the strain formed a tight phylogenetic lineage with Methylomonas koyamae with a value of 99.4% gene sequence homology. So, we named the isolate as Methylomonas sp. SM4. 8.6 mM methanol was accumulated in the reaction mixture containing 70 mM sodium formate and 40 mM MgCl₂ (MDH inhibitor) under atmosphere of methane:air (40:60) mixture for 24 hr at 30℃.

목차

Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES

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UCI(KEPA) : I410-ECN-0101-2018-470-000978031