지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.6
- 수록면
- 124 - 132 (9page)
- DOI
- 10.7841/ksbbj.2017.32.2.124
이용수
초록· 키워드
오류제보하기
A methane-oxidizing bacterium was isolated from rice paddy field soil around Jeollanam-do province, Korea, and characterized. The isolate was gram-negative, orange pigmented and short rod (1.1-1.2 × 1.6-1.9 μm). It was catalase and urease-negative but oxidase-positive. The strain utilized methane and methanol as sole carbon and energy sources. It had an ability to grow with an optimum pH 7.0 and an optimum growth temperature 30℃. The strain was resistant to antibiotic polymyxin B but sensitive to streptomycin, kanamycin, ampicillin, chloramphenicol and rifampicin. The isolate required copper for their growth with concentration range of 2-25 μM, with an optimum of 10 μM. Under optimal culture condition, specific cell growth rate and generation time were found to be 0.046 hr<SUP>-1</SUP> and 15.13 hr, respectively. Phylogenetic analysis based on 16S rDNA sequences indicated that the strain formed a tight phylogenetic lineage with Methylomonas koyamae with a value of 99.4% gene sequence homology. So, we named the isolate as Methylomonas sp. SM4. 8.6 mM methanol was accumulated in the reaction mixture containing 70 mM sodium formate and 40 mM MgCl₂ (MDH inhibitor) under atmosphere of methane:air (40:60) mixture for 24 hr at 30℃.
목차
Abstract
1. INTRODUCTION
2. MATERIALS AND METHODS
3. RESULTS AND DISCUSSION
4. CONCLUSION
REFERENCES
참고문헌 (32)
참고문헌 신청
IPPC (1997) Revised 1996 IPCC Guidelines-3. 1.33-1.35.
Park, S. H. and S. Y. Choo (1993) Production of methanol by resting cells of Methylosinus trichosporium OB3b, Kor. J. Biotechonol. Bioeng. 8: 341-350.
Hong, J. K. Methanol opening the de-petroleum era. http://www.lgeri.com/uploadFiles/ko/pdf/ind/indus938-3_20070525173614.pdf. (2007).
Casey P. S., T. McAllister, and K. Foger (1994) Selective oxidation of methane to methanol at high pressures. Ind. Eng. Chem. Res. 33: 1120-1125.
Corder, R. E., E. R. Johnson, J. L. Vega, E. C. Clausen, and J. L. Gaddy. Biological production of methanol from methane. http://www.anl.gov/PCS/acsfuel/preprint% 20archive/Files/33_3_LOS%20ANGELES_09-88_0469. pdf (1986).
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