지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2017.1
- 수록면
- 31 - 35 (5page)
이용수
초록· 키워드
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Background: Korean ginseng (Panax ginseng) is a well-known medicinal plant of Oriental medicine that is still in practice today. Until now, a total of 11 Korean ginseng cultivars with unique features to Korean ginseng have been developed based on the pure-line-selection method. Among them, a new cultivar namely G-1 with different agricultural traits related to yield and content of ginsenosides, was developed in 2012.
Methods: The aim of this study was to distinguish the new ginseng cultivar G-1 by identifying the unique single-nucleotide polymorphism (SNP) at its 45S ribosomal DNA and Panax quinquefolius region than other Korean ginseng cultivars using multiplex amplification-refractory mutation systemepolymerase chain reaction (ARMS-PCR).
Results: A SNP at position of 45S ribosomal DNA region between G-1, P. quinquefolius, and the other Korean ginseng cultivars was identified. By designing modified allele-specific primers based on this site, we could specifically identified G-1 and P. quinquefolius via multiplex PCR. The unique primer for the SNP yielded an amplicon of size 449 bp in G-1 cultivar and P. quinquefolius. This study presents an effective method for the genetic identification of the G-1 cultivar and P. quinquefolius.
Conclusion: The results from our study shows that this SNP-based approach to identify the G-1 cultivar will be a good way to distinguish accurately the G-1 cultivar and P. quinquefolius from other Korean ginseng cultivars using a SNP at 45S ribosomal DNA region.
Methods: The aim of this study was to distinguish the new ginseng cultivar G-1 by identifying the unique single-nucleotide polymorphism (SNP) at its 45S ribosomal DNA and Panax quinquefolius region than other Korean ginseng cultivars using multiplex amplification-refractory mutation systemepolymerase chain reaction (ARMS-PCR).
Results: A SNP at position of 45S ribosomal DNA region between G-1, P. quinquefolius, and the other Korean ginseng cultivars was identified. By designing modified allele-specific primers based on this site, we could specifically identified G-1 and P. quinquefolius via multiplex PCR. The unique primer for the SNP yielded an amplicon of size 449 bp in G-1 cultivar and P. quinquefolius. This study presents an effective method for the genetic identification of the G-1 cultivar and P. quinquefolius.
Conclusion: The results from our study shows that this SNP-based approach to identify the G-1 cultivar will be a good way to distinguish accurately the G-1 cultivar and P. quinquefolius from other Korean ginseng cultivars using a SNP at 45S ribosomal DNA region.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
References
참고문헌 (27)
참고문헌 신청
[학술지(정기간행물)] - Attele AS / 1999 / Ginseng pharmacology : multiple constituents and multiple actions / Biochem Pharmacol 58 : 1685 ~ 1693
[학술지(정기간행물)] - Chong SK / 1988 / Ginsengdis there a use in clinical medicine? / Postgrad Med J 64 : 841 ~ 846
[학술지(정기간행물)] - Ho Jae Park / 2012 / Ginseng in the traditional herbal prescriptions / Journal of Ginseng Research 36 (3) : 225 ~ 241
[학술지(정기간행물)] - Juyeon Jung / 2014 / Practical application of DNA markers for high-throughput authentication of Panax ginseng and Panax quinquefolius from commercial ginseng products / Journal of Ginseng Research 38 (2) : 123 ~ 129
[학술지(정기간행물)] - Kwon WS / 1998 / Breeding process and characteristics of KG101, a superior line of Panax ginseng C. A. Meyer. / Korean J Ginseng Sci 22 : 11 ~ 17
[학술지(정기간행물)] - Chong SK / 1988 / Ginsengdis there a use in clinical medicine? / Postgrad Med J 64 : 841 ~ 846
[학술지(정기간행물)] - Ho Jae Park / 2012 / Ginseng in the traditional herbal prescriptions / Journal of Ginseng Research 36 (3) : 225 ~ 241
[학술지(정기간행물)] - Juyeon Jung / 2014 / Practical application of DNA markers for high-throughput authentication of Panax ginseng and Panax quinquefolius from commercial ginseng products / Journal of Ginseng Research 38 (2) : 123 ~ 129
[학술지(정기간행물)] - Kwon WS / 1998 / Breeding process and characteristics of KG101, a superior line of Panax ginseng C. A. Meyer. / Korean J Ginseng Sci 22 : 11 ~ 17
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