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논문 기본 정보

자료유형
학술저널
저자정보
Dahae Lee (Sungkyunkwan University) Ki Sung Kang (Gachon University) Jae Sik Yu (Sungkyunkwan University) Jung-Yoon Woo (Korea Ginseng Corporation) Gwi Seo Hwang (Gachon University) Dae-Woon Eom (University of Ulsan) Seung-Hoon Baek (Ajou University) Hye Lim Lee (Gachon University) Ki Hyun Kim (Sungkyunkwan University) Noriko Yamabe (Gachon University)
저널정보
고려인삼학회 Journal of Ginseng Research Journal of Ginseng Research Vol.41 No.3
발행연도
2017.7
수록면
284 - 289 (6page)

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Background: Compound FK506 is an immunosuppressant agent that is frequently used to prevent rejection of solid organs upon transplant. However, nephrotoxicity due to apoptosis and inflammatory response mediated by FK506 limit its usefulness. In this study, the protective effect of Korean Red Ginseng (KRG) against FK506-induced damage in LLC-PK1 pig kidney epithelial cells was investigated.
Methods: LLC-PK1 cells were exposed to FK506 with KRG and cell viability was measured. Western blotting and RT-PCR analyses evaluated protein expression of MAPKs, caspase-3, and KIM-1. TLR-4 gene expression was assessed. Caspase-3 activities were also determined. The number of apoptotic cells was measured using an image-based cytometric assay.
Results: The reduction in LLC-PK1 cell viability by 60mM FK506 was recovered by KRG cotreatment in a dose-dependent manner. The phosphorylation of p38, p44/42 MAPKs (ERK), KIM-1, cleaved caspase-3, and TLR-4 mRNA expression was increased markedly in LLC-PK1 cells treated with 60mM FK506. However, with the exception of p-ERK, elevated levels of p-p38, KIM-1, cleaved caspase-3, and TLR-4 mRNA expression were significantly decreased after cotreatment with KRG. Activity level of caspase-3 was also attenuated by KRG cotreatment. Moreover, image-based cytometric assay showed that apoptotic cell death was increased by 60mM FK506 treatment, whereas it was decreased after cotreatment with KRG.
Conclusion: Taken together, these results suggest that the molecular mechanism of KRG in the FK506-induced nephrotoxicity may lead to the development of an adjuvant for the inhibition of adverse effect FK506 in the kidney.
#FK506 #Korean Red Ginseng #nephrotoxicity #MAPKs

목차

ABSTRACT
1. Introduction
2. Materials and methods
3. Results and discussion
References

참고문헌 (26)

참고문헌 신청
[학술지(정기간행물)] - Wallemacq PE / 1993 / FK506 (tacrolimus), a novel immunosuppressant in organ transplantation: clinical, biomedical, and analytical aspects / Clin Chem 39 : 2219 ~ 2228 google schola [학술지(정기간행물)] - Dumont FJ / 2000 / FK506, an immunosuppressant targeting calcineurin function / Curr Med Chem 7 : 731 ~ 748 google schola [학술지(정기간행물)] - Tada H / 2002 / Effects of thymic hormone on reactive oxygen speciesscavengers and renal function in tacrolimus-induced nephrotoxicity / Life Sci 70 : 1213 ~ 1223 google schola [학술지(정기간행물)] - Cosner D / 2015 / Proximal tubular injury in medullary rays is an early sign of acute tacrolimus nephrotoxicity / J Transplant 2015 : 142521 ~ google schola [학술지(정기간행물)] - Randhawa PS / 1997 / Tacrolimus(FK506)-associated renal pathology / Adv Anat Pathol 4 : 265 ~ 276 google schola

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