지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.6
- 수록면
- 343 - 351 (9page)
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초록· 키워드
오류제보하기
Background: Red ginseng is a popularly used traditional medicine with antiaging effects in Asian countries. The present study aimed to explore the changes in protein expression underlying the mechanisms of life span extension and antiaging caused by red ginseng extract (RGE) in Drosophila melanogaster.
Methods: A proteomic approach of two-dimensional polyacrylamide gel electrophoresis (2-DE) was used to identify the differential abundance of possible target proteins of RGE in D. melanogaster. The reliability of the 2-DE results was confirmed via Western blotting to measure the expression levels of selected proteins. Proteins altered at the expression level after RGE treatment (1 mg/mL) were identified by matrix-assisted laser desorption/ionization-time of flight tandem mass spectrometry and by searching against the National Center for Biotechnology nonredundant and Uniprot protein databases. The differentially expressed proteins were analyzed using bioinformatics methods.
Results: The average survival life span of D. melanogaster was significantly extended by 12.60% with RGE treatment (1 mg/mL) compared to untreated flies. This followed increased superoxide dismutase level and decreased methane dicarboxylic aldehyde content. Based on the searching strategy, 23 differentially expressed proteins were identified (16 up-regulated and 7 down-regulated) in the RGE-treated D. melanogaster. Transduction pathways were identified using the Kyoto Encyclopedia of Genes and Genomes database, and included the hippo and oxidative phosphorylation pathways that play important roles in life span extension and antiaging process of D. melanogaster.
Conclusion: Treatment with RGE in D. melanogaster demonstrated that mechanisms of life span extension and antiaging are regulated by multiple factors and complicated signal pathways.
Methods: A proteomic approach of two-dimensional polyacrylamide gel electrophoresis (2-DE) was used to identify the differential abundance of possible target proteins of RGE in D. melanogaster. The reliability of the 2-DE results was confirmed via Western blotting to measure the expression levels of selected proteins. Proteins altered at the expression level after RGE treatment (1 mg/mL) were identified by matrix-assisted laser desorption/ionization-time of flight tandem mass spectrometry and by searching against the National Center for Biotechnology nonredundant and Uniprot protein databases. The differentially expressed proteins were analyzed using bioinformatics methods.
Results: The average survival life span of D. melanogaster was significantly extended by 12.60% with RGE treatment (1 mg/mL) compared to untreated flies. This followed increased superoxide dismutase level and decreased methane dicarboxylic aldehyde content. Based on the searching strategy, 23 differentially expressed proteins were identified (16 up-regulated and 7 down-regulated) in the RGE-treated D. melanogaster. Transduction pathways were identified using the Kyoto Encyclopedia of Genes and Genomes database, and included the hippo and oxidative phosphorylation pathways that play important roles in life span extension and antiaging process of D. melanogaster.
Conclusion: Treatment with RGE in D. melanogaster demonstrated that mechanisms of life span extension and antiaging are regulated by multiple factors and complicated signal pathways.
목차
ABSTRACT
1. Introduction
2. Materials and methods
3. Results
4. Discussion
5. Conclusions
References
참고문헌 (43)
참고문헌 신청
[학술지(정기간행물)] - Ghimire S / 2017 / Jujube (Ziziphus Jujuba Mill.) fruit feeding extends lifespan and increases tolerance to environmental stresses by regulating agingassociated gene expression in Dros / Biogerontology 18 : 263 ~ 273
[학술지(정기간행물)] - Ahangarpour A / 2016 / Effects of Portulaca oleracea ethanolic extract on reproductive system of aging female mice / Int J Reprod Biomed (Yazd) 14 : 205 ~ 212
[학술지(정기간행물)] - Peleg S / 2016 / Life span extension by targeting a link between metabolism and histone acetylation in Drosophila / EMBO Rep 17 : 455 ~ 469
[학술지(정기간행물)] - Saurav Ghimire / 2015 / Defensive Behavior against Noxious Heat Stimuli Is Declined with Aging Due to Decreased Pain-Associated Gene Expression in Drosophila / Biomolecules & Therapeutics 23 (3) : 290 ~ 295
[학술지(정기간행물)] - Ong WY / 2015 / Protective effects of ginseng on neurological disorders / Front Aging Neurosci 7 : 129 ~
[학술지(정기간행물)] - Ahangarpour A / 2016 / Effects of Portulaca oleracea ethanolic extract on reproductive system of aging female mice / Int J Reprod Biomed (Yazd) 14 : 205 ~ 212
[학술지(정기간행물)] - Peleg S / 2016 / Life span extension by targeting a link between metabolism and histone acetylation in Drosophila / EMBO Rep 17 : 455 ~ 469
[학술지(정기간행물)] - Saurav Ghimire / 2015 / Defensive Behavior against Noxious Heat Stimuli Is Declined with Aging Due to Decreased Pain-Associated Gene Expression in Drosophila / Biomolecules & Therapeutics 23 (3) : 290 ~ 295
[학술지(정기간행물)] - Ong WY / 2015 / Protective effects of ginseng on neurological disorders / Front Aging Neurosci 7 : 129 ~
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UCI(KEPA) : I410-ECN-0101-2018-524-003161226