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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2019.1
- 수록면
- 83 - 91 (9page)
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초록· 키워드
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Nanoparticles (NPs) have been recognized as both useful tools and potentially toxic materials in various industrial and medicinal fields. Previously, we found that zinc oxide (ZnO) NPs that are neurotoxic to human dopaminergic neuroblastoma SH-SY5Y cells are mediated by lipoxygenase (LOX), not cyclooxygenase-2 (COX-2). Here, we examined whether human bone marrow-derived mesenchymal stem cells (MSCs), which are different from neuroblastoma cells, might exhibit COX-2- and/or LOX-dependent cytotoxicity of ZnO NPs. Additionally, changes in annexin V expression, caspase-3/7 activity, and mitochondrial membrane potential (MMP) induced by ZnO NPs and ZnO were compared at 12 hr and 24 hr after exposure using flow cytometry. Cytotoxicity was measured based on lactate dehydrogenase activity and confirmed by trypan blue staining. Rescue studies were executed using zinc or iron chelators. ZnO NPs and ZnO showed similar dose-dependent and significant cytotoxic effects at concentrations ≥ 15 μg/mL, in accordance with annexin V expression, caspase-3/7 activity, and MMP results. Human MSCs exhibited both COX-2 and LOX-mediated cytotoxicity after exposure to ZnO NPs, which was different from human neuroblastoma cells. Zinc and iron chelators significantly attenuated ZnO NPs-induced toxicity. Conclusively, these results suggest that ZnO NPs exhibit both COX-2- and LOX-mediated apoptosis by the participation of mitochondrial dysfunction in human MSC cultures.
목차
Abstract
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES
참고문헌 (29)
참고문헌 신청
Navya, P.N. / 2016 / Rational engineering of physicochemical properties of nanomaterials for biochemical applications with nanotoxicological perspectives / Nano. Converg 3:1~14
Wu, T. / 2018 / Review of the effects of manufactured nanoparticles on mammalian target organs / J. Appl. Toxicol 38:25~40
Dineley, K.E. / 2003 / Zinc inhibition of cellular energy production: implication for mitochondria and neurodegeneration / J. Neurochem 85:563~570
Capasso, M. / 2005 / Zinc dyshomeostasis: a key modulator of neuronal injury / J. Alzheimers Dis 8:93~108
Manzoor, U. / 2016 / Antibacterial, structural and optical characterization of mechano-chemically prepared ZnO nanoparticles / PLoS ONE 11:e0154704
Wu, T. / 2018 / Review of the effects of manufactured nanoparticles on mammalian target organs / J. Appl. Toxicol 38:25~40
Dineley, K.E. / 2003 / Zinc inhibition of cellular energy production: implication for mitochondria and neurodegeneration / J. Neurochem 85:563~570
Capasso, M. / 2005 / Zinc dyshomeostasis: a key modulator of neuronal injury / J. Alzheimers Dis 8:93~108
Manzoor, U. / 2016 / Antibacterial, structural and optical characterization of mechano-chemically prepared ZnO nanoparticles / PLoS ONE 11:e0154704
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UCI(KEPA) : I410-ECN-0101-2019-513-000294527