지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.10
- 수록면
- 362 - 369 (8page)
- DOI
- 10.7783/KJMCS.2018.26.5.362
이용수
초록· 키워드
오류제보하기
Background: For stable induction of tetraploidy in Fallopia multiflora Haraldson, colchicine was treated to establish the condition of induction and investigated the morphological and cytogenetic traits of the tetraploid plants obtained compared to those of diploid ones.
Methods and Results: For the induction of tetraploidy, F. multiflora plants were soaked in aqueous solutions of colchicine at various concentration (0.1, 0.5, and 1.0%). After this, 2% dimethyl sulfoxide (DMSO) was added at room temperature on a shaker set at 150 rpm for periods of 12, 24, and 48 h. The induction rate of tetraploids appeared to be the highest in plants treated with 0.5% colchicine for 24 h. As the colchicine concentration and soaking time increased above these levels, the growing tip of the roots did not develop and they began to rot. When compared to diploid plants, tetraploids differed greatly in various characteristics, including the sizes and shapes of the leaves, fruits, flowers and roots. The induced tetraploid F. multiflora had larger guard cells, and chloroplasts, increased number of chloroplast in the guard cells and decreased stomatal densities.
Conclusions: When colchicine induced plants for tetraploid, it can be distinguished from diploids, in various characteristics such as morphological changes as stomatal size, number of chloroplasts per guard cell, number of chromosomes and flow cytometry. Therefore, it proved that these methods are suitable, quick and easy methods for the identification of the ploidy level of F. multiflora.
Methods and Results: For the induction of tetraploidy, F. multiflora plants were soaked in aqueous solutions of colchicine at various concentration (0.1, 0.5, and 1.0%). After this, 2% dimethyl sulfoxide (DMSO) was added at room temperature on a shaker set at 150 rpm for periods of 12, 24, and 48 h. The induction rate of tetraploids appeared to be the highest in plants treated with 0.5% colchicine for 24 h. As the colchicine concentration and soaking time increased above these levels, the growing tip of the roots did not develop and they began to rot. When compared to diploid plants, tetraploids differed greatly in various characteristics, including the sizes and shapes of the leaves, fruits, flowers and roots. The induced tetraploid F. multiflora had larger guard cells, and chloroplasts, increased number of chloroplast in the guard cells and decreased stomatal densities.
Conclusions: When colchicine induced plants for tetraploid, it can be distinguished from diploids, in various characteristics such as morphological changes as stomatal size, number of chloroplasts per guard cell, number of chromosomes and flow cytometry. Therefore, it proved that these methods are suitable, quick and easy methods for the identification of the ploidy level of F. multiflora.
목차
ABSTRACT
서언
재료 및 방법
결과 및 고찰
REFERENCE
참고문헌 (9)
참고문헌 신청
길진수 / 2017 / SSR 마커를 이용한 산양삼의 유전적 다양성 분석 / 한국약용작물학회지 25(6):389~396
강귀보 / 2016 / GC-MS 기반 대사체학 기술을 응용한 참당귀의 산지비교분석 / 한국약용작물학회지 24(2):93~100
Kim IH / 2003 / Breeding of tetraploid in Platycodon grandiflorum(Jacq.) A.DC. by colchichine treatment / Plant resources 6(3):188~194
김민자 / 2014 / 백수오, 이엽우피소, 박주가리 및 하수오의 형태적 형질 비교 / 한국약용작물학회지 22(2):113~120
고갑천 / 2002 / 무등산 수박의 4배체 작성 / Horticulture, Environment, and Biotechnology 43(6):671~676
강귀보 / 2016 / GC-MS 기반 대사체학 기술을 응용한 참당귀의 산지비교분석 / 한국약용작물학회지 24(2):93~100
Kim IH / 2003 / Breeding of tetraploid in Platycodon grandiflorum(Jacq.) A.DC. by colchichine treatment / Plant resources 6(3):188~194
김민자 / 2014 / 백수오, 이엽우피소, 박주가리 및 하수오의 형태적 형질 비교 / 한국약용작물학회지 22(2):113~120
고갑천 / 2002 / 무등산 수박의 4배체 작성 / Horticulture, Environment, and Biotechnology 43(6):671~676
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