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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2018.2
- 수록면
- 8 - 18 (11page)
- DOI
- 10.7783/KJMCS.2018.26.1.8
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초록· 키워드
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Background: This present study was conducted to evaluate the anti-inflammatory and immune regulatory effects of Aucklandia lappa Decne (AL).
Methods and Results: We measured cytotoxicity, nitric oxide (NO) content, mRNA expression (iNOS, IL-1α, IL-1β, and TNF-α), protein expression (iNOS, COX-2, and IκB-α) and phagocytic activity in RAW264.7 cells. Male BALB/c mice were fed 100 ㎎/㎏ AL (Aucklandia lappa Decneon 70% ethanol extract) and 250 ㎎/㎏ AL for 4 weeks; thereafter, we observed B/T or CD4<SUP> </SUP>/CD8<SUP>+</SUP> lymphocyte subpopulation change, and expression patterns of CD4<SUP>+</SUP> and CD8<SUP>+</SUP> lymphocytes by immunohistochemical staining in mouse splenocytes and/or thymocytes. To determine the experimental concentration of AL, cell viability was measured by MTT assay and tested at 12.5 ㎍/㎖ or less. AL inhibited the levels of NO, lymphokine production (IL-1β, and TNF-α), and mRNA (iNOS, IL-1α, IL-1β, and TNF-α) and protein (iNOS, and COX-2) expression. Additionally, the levels of IκB-α, phagocytic activity, and splenic and thymic T lymphocytes, especially T<SUB>H</SUB> and T<SUB>C</SUB> cells were significantly increased in AL administered mice. The immuno-reactive density of CD4<SUP>+</SUP> and CD8<SUP>+</SUP> lymphocytes was stronger in AL groups than in the normal group. AL stimulated NO, iNOS, and COX-2, and regulated IL-1α, IL-1β, TNF-α, and IκB-α in macrophages treated with LPS (lipopolysaccharide). In addition, AL increased the phagocytic activity of macrophages and the immunity of mouse T (T<SUB>H</SUB>, and T<SUB>C</SUB>) cells.
Conclusions: These results suggested that AL might show anti-inflammatory activity via the suppression of various inflammatory markers and immuno-regulatory activity.
Methods and Results: We measured cytotoxicity, nitric oxide (NO) content, mRNA expression (iNOS, IL-1α, IL-1β, and TNF-α), protein expression (iNOS, COX-2, and IκB-α) and phagocytic activity in RAW264.7 cells. Male BALB/c mice were fed 100 ㎎/㎏ AL (Aucklandia lappa Decneon 70% ethanol extract) and 250 ㎎/㎏ AL for 4 weeks; thereafter, we observed B/T or CD4<SUP> </SUP>/CD8<SUP>+</SUP> lymphocyte subpopulation change, and expression patterns of CD4<SUP>+</SUP> and CD8<SUP>+</SUP> lymphocytes by immunohistochemical staining in mouse splenocytes and/or thymocytes. To determine the experimental concentration of AL, cell viability was measured by MTT assay and tested at 12.5 ㎍/㎖ or less. AL inhibited the levels of NO, lymphokine production (IL-1β, and TNF-α), and mRNA (iNOS, IL-1α, IL-1β, and TNF-α) and protein (iNOS, and COX-2) expression. Additionally, the levels of IκB-α, phagocytic activity, and splenic and thymic T lymphocytes, especially T<SUB>H</SUB> and T<SUB>C</SUB> cells were significantly increased in AL administered mice. The immuno-reactive density of CD4<SUP>+</SUP> and CD8<SUP>+</SUP> lymphocytes was stronger in AL groups than in the normal group. AL stimulated NO, iNOS, and COX-2, and regulated IL-1α, IL-1β, TNF-α, and IκB-α in macrophages treated with LPS (lipopolysaccharide). In addition, AL increased the phagocytic activity of macrophages and the immunity of mouse T (T<SUB>H</SUB>, and T<SUB>C</SUB>) cells.
Conclusions: These results suggested that AL might show anti-inflammatory activity via the suppression of various inflammatory markers and immuno-regulatory activity.
목차
ABSTRACT
서언
재료 및 방법
결과
고찰
REFERENCES
참고문헌 (44)
참고문헌 신청
Adams DO / 1984 / The cell biology of macrophage activation / Annual Review of Immunology 2:283~318
Admas PD / 2001 / Regulation of the retinoblastoma tumor suppressor protein by cyclin/cdks / Biochimica et Biophysica Acta 1471:123~133
Bocca C / 2004 / A sesquiterpene lactone, costunolide, interacts with microtubule protein and inhibits the growth of MCF-7 cells / Chemico-Biological Interactions 147:79~86
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Analytical Biochemistry 72:248~254
Calixto JB / 2004 / Anti-inflammatory compounds of plant origin. Part II: Modulation of pro-inflammatory cytokines, chemokines and adhesion molecules / Planta Medica 70:93~103
Admas PD / 2001 / Regulation of the retinoblastoma tumor suppressor protein by cyclin/cdks / Biochimica et Biophysica Acta 1471:123~133
Bocca C / 2004 / A sesquiterpene lactone, costunolide, interacts with microtubule protein and inhibits the growth of MCF-7 cells / Chemico-Biological Interactions 147:79~86
Bradford MM / 1976 / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Analytical Biochemistry 72:248~254
Calixto JB / 2004 / Anti-inflammatory compounds of plant origin. Part II: Modulation of pro-inflammatory cytokines, chemokines and adhesion molecules / Planta Medica 70:93~103
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