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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국식품영양과학회 Preventive Nutrition and Food Science Preventive Nutrition and Food Science Vol.24 No.2
- 발행연도
- 2019.6
- 수록면
- 187 - 196 (10page)
- DOI
- 10.3746/pnf.2019.24.2.187
이용수
초록· 키워드
오류제보하기
Mitochondria dynamic is regulated by different proteins, maintaining a balance between fission and fusion. An imbalance towards mitochondrial fission has been associated with tumor cell proliferation. The aim of this study was to analyze whether pectin modifies the viability of human colon cancer cells and the expression of proteins involved in mitochondrial fusion and fission. The human colon carcinoma cell line HT29 cells was growth in 10% fetal bovine serum in the absence and presence of pectin. Pectin reduced HT29 cell viability in a concentration-dependent manner, reaching a plateau at 150∼300 μ㏖/L pectin. The presence of 200 μ㏖/L pectin reduced the expression of dynamin-related protein-1 and increased expression of the mitochondrial fusion-associated proteins mitofusin-1 and 2. Expression of cyclin B1, a protein involved in G2/M transition, was found decreased in pectin-incubated HT29 cells. Moreover, expression of p53 protein, the amount of p53 in the nucleous and β-galactosidase activity, which are all biomarkers for cellular senescence, were significantly higher in pectin-incubated HT29 cells than in HT29 cells incubated without pectin. Expression of the protein B-cell lymphoma 2 (Bcl-2) homologous antagonist/killer was increased in response to incubation with pectin. However, incubation with pectin did not affect expression of Bcl-2-associated X protein or Bcl-2, or the caspase-3 activity. Overall, we concluded that pectin reduces the viability of human HT29 colon cancer cells, which is accompanied with a shift in the expression of proteins associated with mitochondrial dynamics towards mitochondrial fusion. Moreover, incubation with pectin favors cellular senescence over apoptosis in HT29 cells.
목차
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
REFERENCES
참고문헌 (61)
참고문헌 신청
Archer SL / 2013 / Mitochondrial dynamics－mitochondrial fission and fusion in human diseases / N Engl J Med 369:2236~2251
Aune D / 2011 / Nonlinear reduction in risk for colorectal cancer by fruit and vegetable intake based on meta-analysis of prospective studies / Gastroenterology 141:106~118
Bagheri E / 2018 / The apoptotic effects of Brucea javanica fruit extract against HT29 cells associated with p53 upregulation and inhibition of NF-κB translocation / Drug Des Devel Ther 12:657~671
Boland ML / 2013 / Mitochondrial dysfunction in cancer / Front Oncol 3:292
Burkitt DP / 1971 / Epidemiology of cancer of the colon and rectum / Cancer 28:3~13
Aune D / 2011 / Nonlinear reduction in risk for colorectal cancer by fruit and vegetable intake based on meta-analysis of prospective studies / Gastroenterology 141:106~118
Bagheri E / 2018 / The apoptotic effects of Brucea javanica fruit extract against HT29 cells associated with p53 upregulation and inhibition of NF-κB translocation / Drug Des Devel Ther 12:657~671
Boland ML / 2013 / Mitochondrial dysfunction in cancer / Front Oncol 3:292
Burkitt DP / 1971 / Epidemiology of cancer of the colon and rectum / Cancer 28:3~13
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UCI(KEPA) : I410-ECN-0101-2019-594-000871021