Changes in the Expression and Distribution of Claudins, Increased Epithelial Apoptosis, and a Mannan-Binding Lectin-Associated Immune Response Lead to Barrier Dysfunction in Dextran Sodium Sulfate-Induced Rat Colitis

Fangyu Wang; Bosi Yuan; Shuping Zhou; Youke Lu; Jiong Liu; Xinxin Jin; Haijun Wan

추천

검색

자료유형: 학술저널

저자정보: Fangyu Wang Bosi Yuan Shuping Zhou Youke Lu Jiong Liu Xinxin Jin Haijun Wan

저널정보: 거트앤리버 발행위원회 Gut and Liver Gut and Liver 제9권 제6호

발행연도: 2015.1

수록면: 734 - 740 (7page)

이용수

📌

연구주제

📖

연구배경

🔬

연구방법

🏆

연구결과

초록· 키워드

오류제보하기

Background/Aims: This animal study aimed to define the underlying cellular mechanisms of intestinal barrier dysfunction. Methods: Rats were fed 4% with dextran sodium sulfate (DSS) to induce experimental colitis. We analyzed the sugars in 24-hour urine output by high pressure liquid chromatography. The expression of claudins, mannan-binding lectin (MBL), and MBL-associated serine proteases 2 (MASP-2) were detected in the colonic mucosa by immunohistochemistry; and apoptotic cells in the colonic epithelium were detected by the terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling method assay. Results: The lactulose and sucralose excretion levels in the urine of rats with DSS-induced colitis were significantly higher than those in the control rats. Mannitol excretion was lower and lactulose/mannitol ratios and sucralose/mannitol ratios were significantly increased compared with those in the control group (p<0.05). Compared with the controls, the expression of sealing claudins (claudin 3, claudin 5, and claudin 8) was significantly decreased, but that of claudin 1 was increased. The expression of pore-forming claudin 2 was upregulated and claudin 7 was downregulated in DSS-induced colitis. The epithelial apoptotic ratio was 2.8%±1.2% in controls and was significantly increased to 7.2%±1.2% in DSS-induced colitis. The expression of MBL and MASP-2 in the intestinal mucosa showed intense staining in controls, whereas there was weak staining in the rats with colitis. Conclusions: There was increased intestinal permeability in DSS-induced colitis. Changes in the expression and distribution of claudins, increased epithelial apoptosis, and the MASP-2-induced immune response impaired the intestinal epithelium and contributed to high intestinal permeability.

#Colitis #ulcerative #Claudin #Intestinal permeability #Apoptosis #Mannan-binding lectin-associated serine proteases 2

참고문헌 (26)

참고문헌 신청

Boniotto M / 2002 / Variant mannose-binding lectin alleles are associated with celiac disease / Immunogenetics 54 : 596 ~ 598

Cader MZ / 2013 / Recent advances in inflammatory bowel disease: mucosal immune cells in intestinal inflammation / Gut 62 : 1653 ~ 1664

Colombel JF / 2008 / The 10 remaining mysteries of inflammatory bowel disease / Gut 57 : 429 ~ 433

Degn SE / 2012 / Mannan-binding lectin-associated serine protease (MASP)-1 is crucial for lectin pathway activation in human serum, whereas neither MASP-1 nor MASP-3 is requ

Esaki M / 2011 / Endoscopic findings under narrow band imaging colonoscopy in ulcerative colitis / Dig Endosc 23 (1) : 140 ~ 142

함께 읽어보면 좋을 논문

논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!