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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 612 - 616 (5page)
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CD44 pre-mRNA includes 20 exons, of which exons 1-5 (C1-C5) and exons 16-20 (C6-C10) are constant exons, whereas exons 6-15 (V1-V10) are variant exons. V6-exon-containing isoforms have been known to be implicated in tumor cell invasion and metastasis. In the present study, we performed a SR protein screen for CD44 V6 splicing using overexpression and lentivirus-mediated shRNA treatment. Using a CD44 V6 minigene, we demonstrate that increased SRSF3 and SRSF4 expression do not affect V6 splicing, but increased expression of SRSF1, SRSF6 and SRSF9 significantly inhibit V6 splicing. In addition, using a constitutive exon-specific primer set, we could not detect alterations of CD44 splicing after SR protein-targeting shRNA treatment. However, using a V6 specific primer, we identified that reduced SRSF2 expression significantly reduced the V6 isoform, but increased V6-10 and V6,8-10 isoforms. Our results indicate that SR proteins are important regulatory proteins for CD44 V6 splicing.
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참고문헌 (27)
참고문헌 신청
Anko ML / 2010 / Global analysis reveals srp20-and srp75-specific mrnps in cycling and neural cells / Nat Struct Mol Biol 17 : 962 ~ 970
Bai Y / 1999 / Control of 3'splice site choice in vivo by asf/sf2 and hnrnp a1 / Nucleic Acids Res 27 : 1126 ~ 1134
Bennett KL / 1995 / Cd44isoforms containing exon v3 are responsible for the presentation of heparin-binding growth factor / J Cell Biol 128 : 687 ~ 698
Boukis LA / 2004 / Ser/arg-rich protein-mediated communication between u1and u2 small nuclear ribonucleoprotein particles / J Biol Chem 279 : 29647 ~ 29653
Busch A / 2012 / Evolution of sr protein and hnrnp splicing regulatory factors / Wiley Interdiscip Rev RNA 3 : 1 ~ 12
Bai Y / 1999 / Control of 3'splice site choice in vivo by asf/sf2 and hnrnp a1 / Nucleic Acids Res 27 : 1126 ~ 1134
Bennett KL / 1995 / Cd44isoforms containing exon v3 are responsible for the presentation of heparin-binding growth factor / J Cell Biol 128 : 687 ~ 698
Boukis LA / 2004 / Ser/arg-rich protein-mediated communication between u1and u2 small nuclear ribonucleoprotein particles / J Biol Chem 279 : 29647 ~ 29653
Busch A / 2012 / Evolution of sr protein and hnrnp splicing regulatory factors / Wiley Interdiscip Rev RNA 3 : 1 ~ 12
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