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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 223 - 230 (8page)
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초록· 키워드
오류제보하기
Ascorbic acid (AsA) is a strong antioxidant/ reducing agent that can be converted to dehydroascorbate (DHA) by oxidation in plants. DHA, a very short-lived chemical, is recycled to AsA by dehydroascorbate reductase (DHAR). Previously, DHAR cDNA was isolated from the hairy roots of the sesame plant, and DHAR-overexpressing transgenic potato plants were generated under the control of the CaMV35S promoter (CaMV35S::DHAR). An increase in transgene expression and ascorbate levels were observed in the transgenic plants. In the present study, proteomic analysis revealed that transgenic plants not only accumulated DHAR in their cells, but also induced several other antioxidant enzyme-related proteins during plant growth.
These results suggest that DHAR is important for stress tolerance via induction of antioxidant proteins, and could improve stress tolerance in transgenic potato plants.
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참고문헌 (27)
참고문헌 신청
Asada K / 1999 / The water-water cycle in chloroplasts: scavenging of active oxygens and dissipation of excess photons / Annu Rev Plant Physiol Plant Mol Biol 50:601~639
Blum H / 1987 / Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels / Electrophoresis 8:93~99
천재안 / 2006 / Comparative Expression and Characterization of Dehydroascorbate Reductase cDNA from Transformed Sesame Hairy Roots using Real-Time RT-PCR / Journal of Plant Biology(한국식물학회지) 49(6):507~512
Chun JA / 2007 / Molecular and biochemical characterizations of dehydroascorbate reductase from sesame (Sesamum indicum L.) hairy root cultures / J Agric Food Chem 55:6067~6073
Conklin PL / 1997 / L-ascorbic acid metabolism in the ascorbate-deficient arabidopsis mutant vtc1 / Plant Physiol 115:1277~1285
Blum H / 1987 / Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels / Electrophoresis 8:93~99
천재안 / 2006 / Comparative Expression and Characterization of Dehydroascorbate Reductase cDNA from Transformed Sesame Hairy Roots using Real-Time RT-PCR / Journal of Plant Biology(한국식물학회지) 49(6):507~512
Chun JA / 2007 / Molecular and biochemical characterizations of dehydroascorbate reductase from sesame (Sesamum indicum L.) hairy root cultures / J Agric Food Chem 55:6067~6073
Conklin PL / 1997 / L-ascorbic acid metabolism in the ascorbate-deficient arabidopsis mutant vtc1 / Plant Physiol 115:1277~1285
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