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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2020.1
- 수록면
- 9 - 19 (11page)
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Dehydroascorbate reductase (DHAR) plays a critical role in the regeneration of ascorbic acid (AsA), and widely involved in plant tolerance to biotic and abiotic stresses. In this study, the IbDHAR3 gene was cloned from sweetpotato cultivar Xushu 18 by RT-PCR. The full-length of this gene was 813 bp which encodes 270 amino acids. The IbDHAR3 protein contained two conserved domains of glutathione S-transferase (GST) and GST-C-DHAR, and one chloroplast transit peptide with 52 amino acids length. Transient expression in tobacco leaf epidermal cells indicated that IbDHAR3 protein is subcellular localized to chloroplast. The qRT-PCR results revealed that the relative expression level of IbDHAR3 in leaves is much higher than that in other tissues, and could be up-regulated by ABA, drought, salinity, and high-temperature stresses. The seed germination rate and root elongation were increased in contrast to wild type under mannitol and NaCl stresses in T3 transgenic Arabidopsis overexpressing IbDHAR3 gene. The soil drought experiments showed that the overexpression of IbDHAR3 gene in Arabidopsis reduced the malondialdehyde (MDA) and the H2O2 content, enhanced the level of AtGR gene expression, superoxide dismutase (SOD), ascorbate peroxidase (APX), DHAR activity, and the AsA content. Therefore, overexpression of IbDHAR3 gene could enhance the ability of scavenging reactive oxygen species such as H2O2 by promoting AsA-glutathione cycle and related antioxidant enzymes system, thereby contributing to increased stress tolerance in Arabidopsis.
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참고문헌 (42)
참고문헌 신청
Bartels D / 2005 / Drought and salt tolerance in plants / Crit Rev Plant Sci 24:23~58
Bindschedler LV / 2010 / Early signalling events in the apoplastic oxidative burst in suspension cultured French bean cells involve cAMP and Ca2+ / New Phytol 151:185~194
Bradford MM / 1976 / A rapid method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72:248~254
Bulley SM / 2009 / Gene expression studies in kiwifruit and gene over-expression in Arabidopsis indicates that GDP-L-galactose guanyltransferase is a major control point of vitamin C biosynthesis / J Exp Bot 60:765~778
Chen Z / 2005 / Increasing tolerance to ozone by elevating foliar ascorbic acid confers greater protection against ozone than increasing avoidance / Plant Physiol 138:1673~1689
Bindschedler LV / 2010 / Early signalling events in the apoplastic oxidative burst in suspension cultured French bean cells involve cAMP and Ca2+ / New Phytol 151:185~194
Bradford MM / 1976 / A rapid method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding / Anal Biochem 72:248~254
Bulley SM / 2009 / Gene expression studies in kiwifruit and gene over-expression in Arabidopsis indicates that GDP-L-galactose guanyltransferase is a major control point of vitamin C biosynthesis / J Exp Bot 60:765~778
Chen Z / 2005 / Increasing tolerance to ozone by elevating foliar ascorbic acid confers greater protection against ozone than increasing avoidance / Plant Physiol 138:1673~1689
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