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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2016.1
- 수록면
- 311 - 316 (6page)
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초록· 키워드
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The efficacy of plant breeding has been enhanced by application of molecular markers in population screening and selection. Pepper (Capsicum annuum L.) is a major staple crop that is economically important with worldwide distribution. It is valued for its spicy taste and medicinal effect. The aim of this study was to discover single nucleotide polymorphisms (SNPs), microsatellite markers information, and percentage sharing through orthologous analysis of pepper-specific pungency- related genes. Here, we report the results of transcriptome analysis and microsatellite markers for four pepper varieties that possess a pungency-related gene. Orthologous analyses was performed to identify species-specific pungency-related genes in pepper, Arabidopsis thaliana L., potato (Solanum tuberosum L.), and tomato (Solanum lycopersicum L.). Advancements in next-generation sequencing technologies enabled us to quickly and cost-effectively assemble and characterize genes to select molecular markers in various organisms, including pepper. We identified a total of 9762, 7302, 8596, and 6886 SNPs for the four pepper cultivars Blackcluster, Mandarine, Saengryeg 211, and Saengryeg 213, respectively. We used 454 GS-FLX pyrosequencing to identify microsatellite markers and tri-nucleotide repeats (54.4%), the most common repeats, followed by di-, hexa-, tetra-, and penta-nucleotide repeats. A total of 5156 (15.9%) pepper-specific pungency-related genes were discovered as a result of orthologous analysis.
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참고문헌 (37)
참고문헌 신청
Altschul SF / 1990 / Basic local alignment search tool / J Mol Biol 215:403~410
Ashrafi H / 2012 / De novo assembly of the pepper transcriptome (Capsicum annuum): a benchmark for in silico discovery of SNPs, SSRs and candidate genes / BMC Genomics 13:571
Barbazuk WB / 2007 / SNP discovery via 454 transcriptome sequencing / Plant J 51:910~918
Bosland PW / 2000 / Peppers: Vegetable and Spice Capsicums / CABI Publishing
Cheung F / 2006 / Sequencing Medicago truncatula expressed sequenced tags using 454 Life Sciences technology / BMC Genomics 7:272
Ashrafi H / 2012 / De novo assembly of the pepper transcriptome (Capsicum annuum): a benchmark for in silico discovery of SNPs, SSRs and candidate genes / BMC Genomics 13:571
Barbazuk WB / 2007 / SNP discovery via 454 transcriptome sequencing / Plant J 51:910~918
Bosland PW / 2000 / Peppers: Vegetable and Spice Capsicums / CABI Publishing
Cheung F / 2006 / Sequencing Medicago truncatula expressed sequenced tags using 454 Life Sciences technology / BMC Genomics 7:272
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