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학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
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연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2014.1
- 수록면
- 169 - 176 (8page)
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초록· 키워드
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A positional scanning synthetic peptide combinatorial library (PS-SCL) was screened in order to identify antimicrobial peptides against the cariogenic oral bacteria, Streptococcus mutans. Activity against Streptococcus gordonii and Aggregatibacter actinomycetemcomitans was also examined. The library was comprised of six sub-libraries with the format O(1-6)XXXXX-NH2, where O represents one of 19 amino acids (excluding cysteine) and X represents equimolar mixture of these. Each sub-library was tested for antimicrobial activity against S. mutans and evaluated for antimicrobial activity against S. gordonii and A. actinomycetemcomitans. The effect of peptides was observed using transmission electron microscopy (TEM). Two semi-mixture peptides, RXXXXN-NH2 (pep-1) and WXXXXN-NH2 (pep-2), and one positioned peptide, RRRWRN-NH2 (pep-3), were identified. Pep-1 and pep-2 showed significant antimicrobial activity against Gram positive bacteria (S. mutans and S. gordonii), but not against Gram negative bacteria (A. actinomycetemcomitans). However, pep-3 showed very low antimicrobial activity against all three bacteria. Pep-3 did not form an amphiphilic α-helix, which is a required structure for most antimicrobial peptides. Pep-1 and pep-2 were able to disrupt the membrane of S. mutans. Small libraries of biochemically-constrained peptides can be used to generate antimicrobial peptides against S. mutans and other oral microbes. Peptides derived from such libraries may be candidate antimicrobial agents for the treatment of oral microorganisms.
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참고문헌 (39)
참고문헌 신청
Schmid B / 2007 / Development of albumin-binding camptothecin prodrugs using a Peptide positional scanning library / Bioconjug Chem 18:1786~1799
최재혁 / 2009 / Identification of Novel Bioactive Hexapeptides Against Phytopathogenic Bacteria Through Rapid Screening of a Synthetic Combinatorial Library / Journal of Microbiology and Biotechnology 19(8):792~802
Armishaw CJ / 2010 / A synthetic combinatorial strategy for developing a-conotoxin analogs as potent a7nicotinic acetylcholine receptor antagonists / J Biol Chem 285:1809~1821
Armishaw CJ / 2013 / Discovery of novel antinociceptive α-conotoxin analogues from the direct in vivo screening of a synthetic mixture-based combinatorial library / ACS Comb Sci 15:153~161
Lam KS / 1991 / A new type of synthetic peptide library for identifying ligand-binding activity / Nature 354:82~84
최재혁 / 2009 / Identification of Novel Bioactive Hexapeptides Against Phytopathogenic Bacteria Through Rapid Screening of a Synthetic Combinatorial Library / Journal of Microbiology and Biotechnology 19(8):792~802
Armishaw CJ / 2010 / A synthetic combinatorial strategy for developing a-conotoxin analogs as potent a7nicotinic acetylcholine receptor antagonists / J Biol Chem 285:1809~1821
Armishaw CJ / 2013 / Discovery of novel antinociceptive α-conotoxin analogues from the direct in vivo screening of a synthetic mixture-based combinatorial library / ACS Comb Sci 15:153~161
Lam KS / 1991 / A new type of synthetic peptide library for identifying ligand-binding activity / Nature 354:82~84
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