임산부의 혈중 엽산과 호모시스틴 수준이 태반세포의 DNA 메틸화에 미치는 영향

박보현; 박종순; 이화영; 하은희; 민정원; 박혜숙; 김영주

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자료유형: 학술저널

저자정보: 박보현 박종순 이화영 하은희 민정원 박혜숙 김영주

저널정보: 대한예방의학회 예방의학회지 예방의학회지 제38권 제4호

발행연도: 2005.1

수록면: 437 - 442 (6page)

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Objectives : DNA methylation is one of the best characterized epigenetic mechanisms that play a regulatory role in genome programming and imprinting during embryogenesis. In this present study, we investigated the association between DNA methylation in the human placenta and the maternal folate and homocysteine concentrations on the Methylenetetrahydrofolatereductase (MTHFR) genetic polymorphism during pregnancy. Methods : We investigated 107 pregnant women who visited Ewha Womanကs University Hospital for prenatal care during their 24~28 weeks-period of gestation. During the second trimester, we measured the serum homocysteine and folate concentrations. The MTHFR 677 genetic polymorphism was determine by performing PCR-RFLP assay. The expression of DNA methylation in the human placentas was estimated by using immunohistochemistry method. Results : Serum folate was negatively correlated with the serum homocysteine concentration for all the MTHFR genotypes. We found positive correlation between the folate concentrations and the DNA methylation in the human placenta (p<0.05). An increasing concentration of homocysteine was associated with reduced DNA methylation in the human placenta. The coefficient value was -2.03 (-3.77, -0.29) on the regression model (p<0.05). Conclusion : These findings suggest that the maternal folate and homocysteine levels along with the MTHFR 677 genetic polymorphism during pregnancy affect the DNA methylation in the human placenta.

#DNA methylation #Epigenetics #Folate #Homocysteine #MTHFR gene polymorphism #Placenta

참고문헌 (29)

참고문헌 신청

/ / Mice deficient in methylenetetrahydrofolatereductase exhibit hyperhomocysteinemia anddecreased methylation capacity 10 : 433 ~ 443

/ / and histone acetylation in IUGR rats 18 (1) : 43 ~ 50

/ 1987 / Determination of free andtotal homocysteine in human plasma by highperformance liquid chromatography withfluorescence detection 422 : 43 ~ 52

/ 1993 / Enzymatic methylesterification of erythrocyte membrane proteinsis impaired in chronic renal failure:evidence forhigh levels of the natural inhibitor Sadenosylhomocys

/ 1994 / Folate deficiency inducedhomocysteinaemia in rats:disruption of Sadenosylmethionine=s co-ordinate regulationof homocysteine metabolism 298 : 415 ~ 419

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