지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2015.1
- 수록면
- 404 - 409 (6page)
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초록· 키워드
오류제보하기
Background: ChromID C. difficile agar (CDIF; bioMérieux, France), a chromogenic medium, allows for the isolation and identification of Clostridium difficile strains within 24 hr regardless of pretreatment of stool specimens with heat or alcohol shock. In the present study, we designed and evaluated a simple procedure for the implementation C. difficile cultures using CDIF medium in a tertiary hospital setting.
Methods: We designed a simple protocol for untreated stool specimens using CDIF medium followed by Gram staining and PRO disc (PRO disc K1532B, Key Scientific Products, USA) testing for the identification of C. difficile in colonies produced on CDIF agar. A total of 1,402 prospectively collected stool specimens from patients with suspected C. difficile infection were tested. The protocol was evaluated by phenotypic or molecular identification of C. difficile using Vitek 2 ANC card (bioMérieux) or 16S rDNA/tpi gene sequencing, respectively.
Results: Of 1,402 stool specimens, 650 isolates were cultured in CDIF. Overall, 235 (36.2%, 235/650) strains could be presumptively identified as C. difficile by using Gram staining and PRO disc testing. Of those, 231 (98.3%, 231/235) isolates were confirmed as true C. difficile by molecular assays.
Conclusions: The use of CDIF combined with Gram staining and PRO disc testing of untreated stool specimens would allow for isolation and accurate identification of C. difficile strains and would be advantageous in reducing the multistep process for C. difficile culture.
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참고문헌 (15)
참고문헌 신청
Borriello SP / 1981 / Simplified procedure for the routine isolation of Clostridium difficile from faeces / J Clin Pathol 34 : 1124 ~ 1127
Boseiwaqa LV / 2013 / Comparison of ChromID C. difficile agar and cycloserine-cefoxitin-fructose agar for the recovery of Clostridium difficile / Pathology 45 : 495 ~ 500
Carroll KC / 2011 / Tests for the diagnosis of Clostridium difficile infection: the next generation / Anaerobe 17 : 170 ~ 174
Carson KC / 2013 / Isolation of Clostridium difficile from faecal specimens--a comparison of chromID C. difficile agar and cycloserine-cefoxitin-fructose agar / J Med Microb
Cohen SH / 2010 / Clinical practice guidelines for Clostridium difficile infection in adults: 2010 update by the society for healthcare epidemiology of America (SHEA) and the
Boseiwaqa LV / 2013 / Comparison of ChromID C. difficile agar and cycloserine-cefoxitin-fructose agar for the recovery of Clostridium difficile / Pathology 45 : 495 ~ 500
Carroll KC / 2011 / Tests for the diagnosis of Clostridium difficile infection: the next generation / Anaerobe 17 : 170 ~ 174
Carson KC / 2013 / Isolation of Clostridium difficile from faecal specimens--a comparison of chromID C. difficile agar and cycloserine-cefoxitin-fructose agar / J Med Microb
Cohen SH / 2010 / Clinical practice guidelines for Clostridium difficile infection in adults: 2010 update by the society for healthcare epidemiology of America (SHEA) and the
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