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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2010.1
- 수록면
- 153 - 165 (13page)
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초록· 키워드
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Objectives : The aims of this study were to investigate the cytoprotective, antioxidative and inflammation genes inhibitory effects of Patriniae Radix on the mouse LLC-PK1 cells (renal epithelial cells).
Methods : The cytoprotective effect of Patriniae Radix was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The antioxidative effect was measured in terms of generation amount of superoxide anion radical (․O2-) by 2',7'-dichlorodihydrofluorescein diacetate (DCFDA), nitric oxide (NO) by 4,5-diaminofluorescein (DAF-2), peroxynitrite (ONOO-) by dihyldrorhodamine 123 (DHR 123) and prostaglandin E2 (PGE2) by PGE2 immunoassay on H2O2-treated LLC-PK1 cells. For measuring of inflammation genes inhibitory effects, western blot was performed to detect IKK-α, phospho-IκB-α, NF-κB (p50, p65), COX-2, iNOS, IL-1β and VCAM-1 protein level in cytosol fractions from LLC-PK1 cells.
Results : Patriniae Radix extract reduced the H2O2-induced cell death and inhibited the amount of H2O2-induced ․O2-, NO, ONOO-, PGE2 generation dose-dependently on the mouse LLC-PK1 cells in vitro. Also Patriniae Radix extract inhibited the expression of IKK-α, phospho-IκB-α, COX-2, iNOS, IL-1β and VCAM-1 genes dose-dependently by means of decreasing activation of NF-κB.
Conclusions : According to above results, it was identified that Patriniae Radix had the cytoprotective, antioxidative and inflammation genes inhibitory effects. So it was suggested that Patriniae Radix would be effective to the treatment for the inflammatory process and inflammation-related diseases.
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참고문헌 (32)
참고문헌 신청
Beckman JS / 1990 / Apparent hydroxyl radical production by peroxynitrite: Implications for endothelial injury from nitric oxide and superoxide / Proc Natl Acad Sci USA. 87
Belvisi M / 1995 / Nitric oxide synthase activity is elevated in inflammatory lung disease in humans / Eur J Pharmacol 283 : 255 ~ 258
Cathcart R / 1983 / Detection of picomole levels of hydroperoxides using a fluorescent dichlorofluorescein fluorescent assay / Anal Biochem. 134 : 111 ~ 116
Del Arco PG / 1997 / Antioxidants and AP-1 activation: a brief overview / Immunibiology 198 : 273 ~ 278
Floyd RA / 1999 / Neuroinflammatory processes are important in neurodegenerative disease: an hypothesis to explain the increased formation of reactive oxygen and nitrogen sp
Belvisi M / 1995 / Nitric oxide synthase activity is elevated in inflammatory lung disease in humans / Eur J Pharmacol 283 : 255 ~ 258
Cathcart R / 1983 / Detection of picomole levels of hydroperoxides using a fluorescent dichlorofluorescein fluorescent assay / Anal Biochem. 134 : 111 ~ 116
Del Arco PG / 1997 / Antioxidants and AP-1 activation: a brief overview / Immunibiology 198 : 273 ~ 278
Floyd RA / 1999 / Neuroinflammatory processes are important in neurodegenerative disease: an hypothesis to explain the increased formation of reactive oxygen and nitrogen sp
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