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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.1
- 수록면
- 694 - 708 (15page)
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Objectives : Jageum-Jung is used as an anti-cancer agent in oriental medicine, but the mechanism by which it induces cell death in cancer cells is still unclear. The purpose of this study was to investigate the effects of Jageum-Jung on apoptosis and cell cycle arrest in HepG2 hepatoma cells.Methods : Various cancer cell lines including HepG2, C6 glioma, SH-SY5Y, PANC-1, and MCF-7 cells, were used. Apoptosis was determined by DAPI nuclei staining and flow cytometry in HepG2 cells treated with various concentrations (from 25 to 200 ㎍/㎖) of H2O extract of Jageum-Jung (JGJ) for 48 hrs. Expression of cell cycle arrest mediators including Rb, p53, p21, cyclin B1, cdk4, and cyclin E proteins were measured by Western blot analysis. To estimate intracellular hydrogen peroxide levels and intracellular nitric oxide levels, HepG2 cells were stained with DCFH-DA dye and DAF dye, subjected on flow cytometric analysis. Results : 1. Jageum-Jung decreased the viability of HepG2 cells in a dose-dependent manner.2. Jageum-Jung induced the catalytic activation of caspase-3 in HepG2 cells.3.. Jageum-Jung increased the intracellular hydrogen peroxide and NO in HepG2 cells.4. Jageum-Jung increased the expression of Rb, p53 and p21 in HepG2 cells.5. Jageum-Jung induced the expression of cyclin B1, cdk4, and cyclin E in HepG2 cells.Conclusions : Taken together, we suggest that Jageum-Jung exhibits cytotoxic effects on HepG2 cells, causing apoptosis and cell cycle arrest. The results showed that Jageum-Jung may do so by regulating the expression of specific target molecules that promote efficient apoptotic cell death following G2/M phase arrest in a dose-dependent manner.
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참고문헌 (22)
참고문헌 신청
/ 1979 / 紫金錠의 解毒效能에 關한 文獻的 考察
/ 1980 / the significance of apoptosis : 251 ~ 306
/ 1982 / distinct modes of cell death with fundamentally different significance : 229 ~ 2 59
/ 1994 / 抗癌劑 및 放射線 副作用에 대한 韓方療法 9 : 239 ~ 64
/ 1995 / 11q13 amplification in local recurrence of human primary breast cancer 12 : 128 ~ 133
/ 1980 / the significance of apoptosis : 251 ~ 306
/ 1982 / distinct modes of cell death with fundamentally different significance : 229 ~ 2 59
/ 1994 / 抗癌劑 및 放射線 副作用에 대한 韓方療法 9 : 239 ~ 64
/ 1995 / 11q13 amplification in local recurrence of human primary breast cancer 12 : 128 ~ 133
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