사람 구강암종 세포주에서 Interleukin-4에 의해 유도된15-Lipoxygenase-1을 매개로 한 세포고사 작용

김경수; 이정권; 이근완; 윤주헌; 서형석; 김정홍

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자료유형: 학술저널

저자정보: 김경수 이정권 이근완 윤주헌 서형석 김정홍

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제48권 제12호

발행연도: 2005.1

수록면: 1,512 - 1,518 (7page)

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Background and Objectives：In oral cavity cancer (OCC) cells, the effects of interleukin-4 (IL-4) are various according to the cell specificity. However, if IL-4 induces apoptosis on OCC cells, the mediator of this apoptosis is uncertain. Therefore, we investigated whether apoptosis of OCC cells occurs by IL-4 and whether 15-lipoxygenase-1 (15-LO-1) induced by IL-4 is the possible mediator of this apoptosis. Materials and Methods：SCC 1483 cells were used. Flow cytometry and poly ADP-ribose polymerase cleavage were used to examine apoptosis. Western blot analysis and reverse transcription-polymerase chain reaction were used to measure 15-LO-1 protein and mRNA. Results：The inhibition of cell proliferation by more than 50% was noted from 10 ng/ml of IL-4. At this dose, apoptosis was observed and this apoptosis was inhibited by 2.2 μM caffeic acid. 15-LO-1 expression was observed from the 8 hour treatment of IL-4 and apoptosis increased after the 24 hour treatment of IL-4. In this apoptosis, caspase cascade, cyclooxygenase-2, and non-steroidal anti-inflammatory drugs-activated gene-1 (NAG-1) were not involved. Conclusion：IL-4 induced apoptosis in SCC 1483 OCC cells and 15-LO-1 induced by IL-4 may mediate this apoptotic pathway.

#구강암·세포고사·Interleukin-4·15-Lipoxygenase-1. #Interleukin-4·Arachidonate 15-Lipoxygenase·Apoptosis·Mouth Neoplasms.

참고문헌 (20)

참고문헌 신청

/ 1992 / Inhibition of colon and breast carcinomacell growth by interleukin-4 52 : 275 ~ 9

/ 1994 / Inductionof 15-lipoxygenase by interleukin-13 in human blood monocytes 269 : 27631 ~ 4

/ 1996 / Growth stimulation of human head and neck squamous cellcarcinoma cell lines by interleukin 4 2 : 127 ~ 35

/ 1998 / express interleukin-4 receptors in situ : 45 ~ 8

/ 1999 / Expression of15-lipoxygenase-1 in human colorectal cancer 59 : 360 ~ 6

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