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김철호; 임혜진; 강성운; 이하늘; 황혜숙; 윤용로; 김동현

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자료유형: 학술저널

저자정보: 김철호 임혜진 강성운 이하늘 황혜숙 윤용로 김동현

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제51권 제2호

발행연도: 2008.1

수록면: 163 - 170 (8page)

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Aberrant activation of hepatocyte growth factor (HGF) and its receptor, c-Met, has been known to be involved in many human cancer development and progression. During the search for an effective molecule inhibitor of HGF/ c-Met signaling, we have found that Epigallocatechin-3-gallate (EGCG) in green tea might inhibit HGF/c-Met signaling. Studies were performed to address whether EGCG inhibited HGF-dependent tumor proliferation and invasion in HNSCC. Materials and Method：For EGCG inhibition of HGF/c-Met signaling, Western blot was performed. The proliferation of FaDu cells was assayed by counting the number of the cells after treatment by HGF 0, 10 ng/ml, EGCG 1 μM, EGCG 10 μM, HGF 10+EGCG 1 μM, HGF 10+EGCG 10 μM. The dispersion of cells was observed by measuring the separation and morphologic changes of the cells after treatment with HGF 0, 10 ng/ml HGF 10+EGCG 1 μM, HGF 10+EGCG 10 μM for 24 hours. Tumor cell migration was assessed by wound healing assay and tumor cell invasiveness was assessed by the membrane invasion assay. Results：HGF treatment induced rapid activation of c-Met and EGCG inhibited HGF-induced c-Met signaling in FaDu cells. HGF significantly enhanced the growth of HNSCC cells and this phenomenon was inhibited by EGCG in a dose-dependant manner (p<0.05). EGCG inhibited HGF-induced scattering, migration, and invasion of HNSCC cells in a dose-dependent manner (p<0.05). Conclusion：Inhibition of HGF/Met by EGCG leads to decreased proliferation, scattering, migration and invasion in vitro, suggesting the possible use of EGCG in HNSCC associated with down-regulation of HGF/Met signaling. (Korean J Otorhinolaryngol- Head Neck Surg 2008;51:163-70)

#Hepatocyte growth factor·c-Met·Hypopharyngeal cancer·Epigallocatechin-3-gallate #EGCG).

참고문헌 (19)

참고문헌 신청

Adhami VM / 2004 / Oral consumption of green tea polyphenols inhibits insulin-like growth factor- I-induced signaling in an autochthonous mouse model of prostate cancer / Ca

Berger SJ / 2001 / Green tea constituent (--)-epigallocatechin-3-gallate inhibits topoisomerase I activity in human colon carcinoma cells / Biochem Biophys Res Commun 288 (1)

Bottaro DP / 1991 / Vande Woude GF, et al. Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product / Science 251 (4995) : 802 ~ 804

Crespy V / 2004 / A review of the health effects of green tea catechins in in vivo animal models / J Nutr 134 (12) : 3431 ~ 3440

Fang MZ / 2003 / Tea polyphenol (--)-epigallocatechin-3-gallate inhibits DNA methyltransferase and reactivates methylation-silenced genes in cancer cell lines / Cancer Res 63

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