인간 유양돌기 골수 유래 간엽줄기세포의 획득 및 신경세포로의 분화

조형호; 정한성; 장수정; 박종성; 조휴채; 장철호; 조용범

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자료유형: 학술저널

저자정보: 조형호 정한성 장수정 박종성 조휴채 장철호 조용범

저널정보: 대한이비인후과학회 대한이비인후-두경부외과학회지 대한이비인후과학회지 두경부외과학 제51권 제5호

발행연도: 2008.1

수록면: 422 - 428 (7page)

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Reports of neural differentiation of mesenchymal stem cells suggest the possibility that these cells may serve as a source for stem cell-based regenerative medicine to treat neurological disorders. The purpose of this study was to generate neural cells by differentiation of bone marrow-derived mesenchymal stem cells that isolated from human mastoid process. Materials and Method：Human mesenchymal stem cells (hMSCs) isolated from human mastoid process bone marrow during mastoidectomy for chronic otitis media surgery were characterized using fluorescence-activated cell sorter. Induction of neural differentiation from hMSCs was performed using mitogenic factors (basic fibroblast growth factor, epidermal growth factor, forskolin, isobutylmethylxanthine), and the characterization of differentiated hMSCs was performed using immunohistochemistry, RT-PCR and whole cell patch clamp technique. Results：hMSCs from bone marrow of mastoid process were isolated and cultured. Differentiated cells from hMSCs expressed mRNA transcripts for neuron specific markers, TUJ1 and neurofilament proteins (NF-L, NF-M) as determined by RT-PCR, and neuron specific markers, suhc as NeuN, TUJ1, microtubule- associated protein-2 (MAP2) and glial fibrillary acidic protein by immunohistochemistry. These cells showed voltagedependent sodium currents that was blocked by tetrodotoxin. Conclusion：hMSCs, which were isolated from human mastoid process bone marrow, were one of the good sources for stem cell-based regenerative medicine to treat neurological disorders. (Korean J Otorhinolaryngol-Head Neck Surg 2008;51:422-8)

#Mesenchymal stem cells· Cell diferentiation· Temporal bone· Mastoid.

참고문헌 (19)

참고문헌 신청

Alvarez-Dolado M / 2003 / Fusion of bone-marrow-derived cells with Purkinje neurons, cardiomyocytes and hepatocytes / Nature 425 (6961) : 968 ~ 973

Barberi T / 2003 / Neural subtype specification of fertilization and nuclear transfer embryonic stem cells and application in parkinsonian mice / Nat Biotechnol 21 (10) : 12

Bosch M / 2004 / Cattaneo E, Alberch J, et al. Induction of GABAergic phenotype in a neural stem cell line for transplantation in an excitotoxic model of Huntington’s disea

Cho KJ / 2005 / Neurons derived from human mesenchymal stem cells show synaptic transmission and can be induced to produce the neurotransmitter substance P by interleukin-1

Deng W / 2001 / In vitro differentiation of human marrow stromal cells into early progenitors of neural cells by conditions that increase intracellular cyclic AMP / Biochem

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