지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 13 - 20 (8page)
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초록· 키워드
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Objective : This study developed species-specific PCR and PCR-RFLP to detect the adulteration of Fel ursi products with cattle and pig bile juices.
Methods : All the primers for PCR and PCR-RFLP in this study were designed based on nucleotide sequences of cytochrome b genes in the mitochondria.
Results : The species-specific PCR amplified a DNA fragment of 214, 214, 295, and 167 bp from Fel ursi product, bear fur, cattle bile juice, and pig bile juice, respectively. The survey using the species-specific PCR indicated that some of commercial Fel ursi products were adulterated with cattle and pig bile juices. PCR-RFLP using the restriction endonucleases, HaeIII and HinfI enabled differentiation among Fel ursi product, cattle bile juice, and pig bile juice. Bear furs from two animals showed variations in PCR-RFLP patterns with HaeIII.
Discussion : The detection methods of the species-specific PCR and PCR-RFLP could be useful in eliminating adulterated Fel ursi products from the market.
목차
등록된 정보가 없습니다.
참고문헌 (22)
참고문헌 신청
Acquaah G / 2004 / Understading biotechnology an integrated and cyber-based approach / Pearson Prentice Hall
Bellagamba F / 2001 / Identification of species in animal feedstuffs by polymerase chain reaction-restriction fragment length polymorphism analysis of mitochondrial DNA / J
Delisle I / 2002 / Conserved primers for rapid sequencing of the complete mitochondrial genome from carnivors applied to three species of bears / Mol Biol Evol 19 : 357 ~ 36
Dieffenbach CW / 2003 / PCR primer a laboratory manual / Cold Spring Harbor
Herman L / 2001 / Determination of the animal origin of raw food by species-specific PCR / J Dairy Res 68 : 429 ~ 435
Bellagamba F / 2001 / Identification of species in animal feedstuffs by polymerase chain reaction-restriction fragment length polymorphism analysis of mitochondrial DNA / J
Delisle I / 2002 / Conserved primers for rapid sequencing of the complete mitochondrial genome from carnivors applied to three species of bears / Mol Biol Evol 19 : 357 ~ 36
Dieffenbach CW / 2003 / PCR primer a laboratory manual / Cold Spring Harbor
Herman L / 2001 / Determination of the animal origin of raw food by species-specific PCR / J Dairy Res 68 : 429 ~ 435
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