지원사업
학술연구/단체지원/교육 등 연구자 활동을 지속하도록 DBpia가 지원하고 있어요.
커뮤니티
연구자들이 자신의 연구와 전문성을 널리 알리고, 새로운 협력의 기회를 만들 수 있는 네트워킹 공간이에요.
논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2006.1
- 수록면
- 385 - 392 (8page)
이용수
초록· 키워드
오류제보하기
To develop an inducible expression system, the enhanced artificial nuclear receptors and target reporters were constructed. Artificial nuclear re-ceptors were generated by fusing three domains, consisting of DNA-binding domain (DBD) of GAL4, ligand binding domain (LBD) of progesterone or estrogen receptor, and activation domain (AD) of VP16, sterol regulatory element binding protein (SREBP)-1a, or SREBP-2. The activation domain of tent transcriptional activity. The maximal level of target reporter gene expression was extremely elevated by the usage of ATP citrate-lyase (ACL) minimal promoter -60/+67 in place of artificial TATA promoter, while the SV40 enhancer severely increased the basal transcription in the absence of ligand. The induction system, developed in the present study, was applied to cell therapy, resulting in successful induction of single-chain insulin analogue (SIA) gene expression to correct the hyperglycemia in diabetic animals. By means of subcutaneous cell therapy, the SIA gene expression rapidly occurred after the local topical application of ligand. These results suggest that our system represents a powerful tool for transcriptional can be used for diverse applications, ranging frombasic research to genetherapy.
목차
등록된 정보가 없습니다.
참고문헌 (19)
참고문헌 신청
/ / A selective tran-scriptional induction system for mammalian cells based on Gal4-estrogen receptor fusion proteins : 1657 ~ 61
/ / Chimeric mamma-lian transactivator based on the lac repressor that is regulated by temperature and isopropyl beta-D-thiogalactopyranoside : 5072 ~ 76
/ / Citrate cleavage in adipose tissue : 899 ~ 904
/ / Regulation of ATP-citrate lyase at transcriptional and post-transcriptional levels in rat liver : 264 ~ 71
/ / Tight control of gene expression in mam-malian cells by tetracycline-responsive promoters : 5547 ~ 51
/ / Chimeric mamma-lian transactivator based on the lac repressor that is regulated by temperature and isopropyl beta-D-thiogalactopyranoside : 5072 ~ 76
/ / Citrate cleavage in adipose tissue : 899 ~ 904
/ / Regulation of ATP-citrate lyase at transcriptional and post-transcriptional levels in rat liver : 264 ~ 71
/ / Tight control of gene expression in mam-malian cells by tetracycline-responsive promoters : 5547 ~ 51
함께 읽어보면 좋을 논문
논문 유사도에 따라 DBpia 가 추천하는 논문입니다. 함께 보면 좋을 연관 논문을 확인해보세요!
-
Effects of Co-Expression of Liver X Receptor β-Ligand Binding Domain with its Partner, Retinoid X Receptor α-Ligand Binding Domain, on their Solubility and Biological Activity in Escherichia coli
Journal of Microbiology and Biotechnology
2015 .01
-
Sequencing Analysis of Ligand Binding Domain of Aryl Hydrocarbon Receptor which is a Dioxin Sensitive Gene in the Ornamental Birds
한국조류학회지
2015 .12
-
The Construction of Regulatory Network for Insulin-Mediated Genes by Integrating Methods Based on Transcription Factor Binding Motifs and Gene Expression Variations
Genomics & informatics
2015 .01
-
Transcriptional Regulation and Post-Transcriptional Expression of ADD1/SREBP1c by Insulin
한국동물학회 학술대회
2001 .01
-
Single and Dual Ligand Effects on Gene Expression Changes in Mouse Macrophage Cells
Genomics & informatics
2006 .01
-
Development of Structural Genomics Tool Based on Protein-Ligand Binding Structures
한국유전체학회 Conference
2005 .01
-
고등식물의 유전자 발현의 조절
한국식물학회 심포지움
1987 .01
-
Promoter Structure and Transcriptional Activity of Human Complement Receptor Type I (CR1) Gene
Korean journal of biological sciences
2003 .01
-
Ligand Recognition by the Toll-like Receptor Family
Animal cells and systems
2009 .01
-
The Application of Dual Suicide Gene in Targeted Gene Therapy
한국동물학회 학술대회
1997 .01
-
CLONING OF A RAT $\mu$(mu) RECEPTOR GENE
한국동물학회 학술대회
1995 .01
-
Transcriptional Activation of the Human Resistin Gene by ADD1/SREBP1c
한국동물학회 학술대회
2001 .01
-
Plant Telomere Binding Proteins Have Expanded DNA Binding Domain
한국동물학회 학술대회
2003 .01
-
Transcriptional targeting of gene expression in breast cancerby the promoters of protein regulator of cytokinesis 1 andribonuclease reductase 2
Experimental and Molecular Medicine
2008 .01
-
Improved expression by cytomegalovirus promoter/enhancer and behavior of vascular endothelial growth factor gene after myocardial injection of naked DNA
Experimental and Molecular Medicine
2002 .01
-
Transcriptional activation of insulin-like growth factor binding protein 6 by 17β-estradiol in SaOS-2 cells
Experimental and Molecular Medicine
2009 .01
-
Development of gene therapy for Inuslin-Dependent Diabetes Mellitus(type I, IDDM) by transfer of human pro-insulin gene to skeletal muscle
한국동물학회 학술대회
2001 .01
-
Detection of Transcriptional Activation Domain from Drosophila Twist Gene
한국동물학회 학술대회
1995 .01
-
Characterization of the Regulatory Elements and Their Binding Factor(s) in Bovine Growth Hormone Gene
한국동물학회 학술대회
1995 .01
-
Cilostazol inhibits insulin-stimulated expression of sterol regulatory binding protein-1c via inhibition of LXR and Sp1
Experimental and Molecular Medicine
2014 .01
이 논문의 저자 정보
최근 본 자료
댓글(0)
0