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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2007.1
- 수록면
- 14 - 26 (13page)
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Primary neuronal culture is a powerful tool to study neuronal development, aging, and degeneration. However, cultured neurons show signs of cell death after 2 or 3 weeks. Although the mechanism un-derlying this phenomenon has not been elucidated, several preventive methods have been identified. Here we show that the neuronal loss in primary cortical culture involves calpain activation and subsequent neuronal cell death. Neuronal loss during cultivation showed destruction of neurites and synapses, and a decrease in neuron numbers. -Calpain and m-calpain were initially activated and accumulated by increased RNA expression. This neuronal death exhibited neurodegenerative fea-important in the developmental process and in the pathogenesis of Al zheimers disease. But, postnatal and aged rat cortex did not show calpain activation and prolonged processing of p35 to p25, in contrast to the long-term culture of cortical neurons. In addition, the inhibition of calpains by ALLM or ALLN blocked the conversion of p 35 to p25, indicating that the calpain activity is essential for the neurode-generative features of cell death. Taken together, this study shows that the neuronal loss in primary cortical cultures involves neurodegeneration-like cell death through the activation of calpains and the sub-sequent processing of p35 to p25, but not develop-mental apoptosis or aging. Our results suggest that the long term primary culture of cortical neurons represent a valuable model of neurodegeneration, such as Alzheimers disease.
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참고문헌 (27)
참고문헌 신청
Aguzzi A / 1996 / Transgenic and knockout mice in the study of neurodegenerative diseases 74 : 11 ~ 26
An WG / 2000 / Protease inhibitor-induced apoptosis: accumulation of p53, p21WAF1/ CIP1, and induction of apoptosis are independent markers of proteasome inhibition 14 : 1276
Ando Y / / Murachi T. Purification and characterization of calpains from pig epidermis and their action on epidermal keratin. J Invest Dermatol 1988;90 : 26 ~ 30
Banker GA / / Cowan WM. Rat hippocampal neurons in dispersed cell culture. Brain Res 1977 : 397 ~ 42
Bernath E / 2006 / Elevation of cytoskeletal protein breakdown in aged Wistar rat brain 27 : 624 ~ 632
An WG / 2000 / Protease inhibitor-induced apoptosis: accumulation of p53, p21WAF1/ CIP1, and induction of apoptosis are independent markers of proteasome inhibition 14 : 1276
Ando Y / / Murachi T. Purification and characterization of calpains from pig epidermis and their action on epidermal keratin. J Invest Dermatol 1988;90 : 26 ~ 30
Banker GA / / Cowan WM. Rat hippocampal neurons in dispersed cell culture. Brain Res 1977 : 397 ~ 42
Bernath E / 2006 / Elevation of cytoskeletal protein breakdown in aged Wistar rat brain 27 : 624 ~ 632
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