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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 발행연도
- 2009.1
- 수록면
- 355 - 360 (6page)
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In this study, the arylsulfatase gene (astA, 984 bp ORF) from Pseudoalteromonas carrageenovora genome was expressed on the cell
surface of S. cerevisiae by fusing with Aga2p linked to the membrane anchored protein, Aga1p. The constructed plasmid, pCTAST (7.1 kb), was introduced to S. cerevisiae EBY100 cell, and yeast transformants on
YPDG plate showed the hydrolyzing activity for 4-methylumbelliferyl-sulfate and p-nitrophenyl-sulfate.
When S. cerevisiae EBY100/pCTAST was grown on YPDG medium, the arylsulfatase activity of cell pellet reached about 1.2 unit/mL, whereas no extracellular arylsulfatase activity was detected. The DNA ladder in
agarose prepared from agar by this recombinant arylsulfatase showed similar resolution and migration patterns with a commercial agarose. This results revealed that arylsulfatase expressed on the cell surface of S.
cerevisiae could be applicable to the economic production of electrophoretic-grade agarose.
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참고문헌 (25)
참고문헌 신청
Akagawa-Matsushita, M / 1992 / Alteromonas atlantica sp. nov. and Alteromonas carrageenovora sp. nov., bacteria that decompose algal polysaccharides / Int. J. Syst. Bacteriol
Allan, G / 1971 / Marine polymers; Part 1. A new procedure for the fractionation of agar / Carbohyd. Res. 17 : 234 ~ 236
BYUN, DAE-SEOK / 2004 / Isolation and Characterization of Marine Bacterium Producing Arylsulfatase / Journal of Microbiology and Biotechnology 14 (6) : 1134 ~ 1141
Barbeyron, T / 1995 / Arysulphatase from Alteromonas carrageenovora / Microbiology 141 : 2897 ~ 2904
Boder, E. T / 1997 / Yeast surface display for screening combinatorial polypeptide libraries / Nat. Biotechnol 15 : 553 ~ 557
Allan, G / 1971 / Marine polymers; Part 1. A new procedure for the fractionation of agar / Carbohyd. Res. 17 : 234 ~ 236
BYUN, DAE-SEOK / 2004 / Isolation and Characterization of Marine Bacterium Producing Arylsulfatase / Journal of Microbiology and Biotechnology 14 (6) : 1134 ~ 1141
Barbeyron, T / 1995 / Arysulphatase from Alteromonas carrageenovora / Microbiology 141 : 2897 ~ 2904
Boder, E. T / 1997 / Yeast surface display for screening combinatorial polypeptide libraries / Nat. Biotechnol 15 : 553 ~ 557
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