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논문 기본 정보
- 자료유형
- 학술저널
- 저자정보
- 저널정보
- 한국미생물생명공학회 Journal of Microbiology and Biotechnology Journal of Microbiology and Biotechnology 제14권 제3호
- 발행연도
- 2004.1
- 수록면
- 599 - 606 (8page)
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초록· 키워드
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Gram-positive antagonistic bacilli were isolatedfrom agricultural soils for possible use in biocontrol of plantpathogenic fungi, Fusarium oxysporum, Rhizoctonia solani,and/or Pythium ultimum. Among the 65 antagonistic Grampositivesoil isolates, 22 strains were identified as Bacillusspecies by 16S rDNA sequence analyses. Four strains,including DF14, especially exhibited multiple antagonisticproperties against the three damping-off fungi. Genotypicproperties of the Bacillus isolates were characterized by rapidmolecular fingerprinting methods using repetitive extragenicpalindromic-PCR (REP-PCR), ribosomal intergenic spacerlengthpolymorphisms (RIS-LP), 16S rDNA PCR-restrictionfragment length polymorphisms (PCR-RFLP), and strainspecificPCR assays. The results indicated that the REP-PCRmethod was more valuable than the RIS-LP and 16S rDNAPCR-RFLP analyses as a rapid and reliable approach forbacilli typing and identification. The use of strain-specificprimers designed based on 16S rDNA sequence comparisonsenabled it to be possible to selectively detect a strain, DF14,which is being used as a biocontrol agent against damping-offfungi.
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참고문헌 (26)
참고문헌 신청
Appl / 1992 / Use of repetitive sequences and the polymerase chain reaction to fingerprintthe genomes of Rhizobium meliloti isolates and other soilbacteria : 2180 ~ 2187
Bouzar, H / 1999 / Multiphasic analysis of xanthomonadscausing bacterial spot disease on tomato and pepper in theCaribbean and Central America: Evidence for commonlineages wi
Cartwright / 1995 / PCR amplification of rRNA intergenicspacer regions as a method for epidemiologic typing ofClostridium difficile 33 : 184 ~ 187
Chang, C. E / 2002 / Molecular identification ofvaginal Lactobacillus spp. isolated from Korean women 12 (2) : 312 ~ 317
Crawford / 1993 / Isolation and characterization of actinomyceteantagonists of a fungal root pathogen : 3899 ~ 3905
Bouzar, H / 1999 / Multiphasic analysis of xanthomonadscausing bacterial spot disease on tomato and pepper in theCaribbean and Central America: Evidence for commonlineages wi
Cartwright / 1995 / PCR amplification of rRNA intergenicspacer regions as a method for epidemiologic typing ofClostridium difficile 33 : 184 ~ 187
Chang, C. E / 2002 / Molecular identification ofvaginal Lactobacillus spp. isolated from Korean women 12 (2) : 312 ~ 317
Crawford / 1993 / Isolation and characterization of actinomyceteantagonists of a fungal root pathogen : 3899 ~ 3905
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PCR-RFLP and Sequence Analysis of the rDNA ITS Region in the Fusarium spp.
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Ribosomal DNA의 PCR-RFLP에 의한 국내산 Rhizoctonia solani 균주들의 종내그룹의 구분
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Isolation and Characterization of Bacillus sp. Producing Broad-Spectrum Antibiotics Against Human and Plant Pathogenic Fungi
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Identification of Microorganisms Responsible for Anaerobic Degradation of Pentachlorophenol Using RFLP Analysis of PCR Amplified 16S rDNAs
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Antagonistics against Pathogenic Fusarium solani and Fusarium oxysporum by Novel Peptides from Bacillus amyloliquefaciens PT14
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